Main description:

In the seven years since the publication of Principles and Practice of Bioanalysis bioanalytical methods have remained the same, but their usage patterns have changed. This second edition of a bestseller provides an updated guide to the techniques used in developing and running ultra-trace analyses for drugs, metabolites, and other substances.

Extensively revised, this volume reflects the dynamic growth in methodologies that has occurred over the past five years. Each chapter has been overhauled or re-written to reflect modern practice. Experts in the field offer a logical path through the problems of small molecule bioanalysis, enabling you to choose the appropriate methods of analysis across a number of fields, including the agrochemical and pharmaceutical industries. They cover a wide range of analytical methods, including mass spectrometry, liquid chromatography, immunoassay, NMR, and gas chromatography.

Presenting the information in an informal and highly accessible style, the book covers the theory and practice of bioanalysis, enabling you to gain maximum benefit and to optimize analytical methods. With practical advice and guidance, it delineates how to develop robust analytical methods for a range of compounds, both natural and synthetic. Using these insights, you can more easily recognize problems and quickly solve them.

Contents:

Physico-chemical Properties of Drugs and Metabolites and their Extraction from Biological Material. Solid Phase Extraction. Basic HPLC Theory and Practice. HPLC Optimisation. HPLC Detectors. Gas Chromotography: What It Is and How We Use It. Thin Layer Chromotography. Capillary Electrophoresis. Immunoassay Techniques. Automation of Sample Preparation. Fundamental Aspects of Mass Spectrometry . Applications of Mass Spectrometry. Quantitative Mass Spectometry. Mass Spectrometric Identification of Metabolites. Nuclear Magnetic Resonance in Drug Metabolism. Strategy in Metabolite Isolation and Identificaiton. Strategy for the Development of Quantitative Analytical Procedures.