Main description:

This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds

Contents:

Simplified Synthetic Antibody Libraries
Generation of Dual Variable Domain Immunoglobulin Molecules for Dual-specific Targeting

Discovery of internalizing antibodies to tumor antigens from phage libraries

Optimizing properties of anti-receptor antibodies using kinetic computational models and experiments

Cell-specific siRNA delivery by peptides and antibodies

Conjugation of Anticancer Drugs Through Endogenous Monoclonal Antibody Cysteine Residues

Building and characterizing antibody-targeted lipidic nanotherapeutics

Cell-Targeting Fusion Constructs Containing Recombinant Gelonin

Stapled Peptides for Intracellular Drug Targets

Mapping of vascular zip codes by phage display

Engineering cyclic peptide toxins

Peptide Discovery using Bacterial Display and Flow Cytometry

Oral enzyme therapy for celiac sprue

Rational Design and Evaluation of Mammalian Ribonuclease Cytotoxins

Engineering Reduced-Immunogenicity Enzymes for Amino Acid Depletion Therapy in Cancer

Designed Ankyrin Repeat Proteins (DARPins): From research to therapy

Target-binding proteins based on the tenth human fibronectin type III domain (10Fn3)

Anticalins: small engineered binding proteins based on the lipocalin scaffold

T Cell Receptor Engineering

Engineering Knottins as Novel Binding Agents

Re-Engineering Biopharmaceuticals for Targeted Delivery Across the Blood-Brain Barrier

Practical Theoretic Guidance for the Design of Tumor Targeting Agents

Fusions of elastin-like polypeptides to pharmaceutical proteins

Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells