Main description:

Methods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophysical methods to study lanthanide-protein interactions, Genetically encoded sensors to study lanthanide biology, Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes, Lanthanide-based probes for amino acid modifications, In vitro selection and application of lanthanide-dependent DNAzymes, LRET biosensors for imaging protein interactions in living cells, Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor, EPR spectroscopy of lanthanides, Macromolecular crystallography for f-element complex characterization, and much more.

Contents:

Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs

Tomoyuki Nakagawa

Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor

Eric Schelter

Activity assays of methanol dehydrogenases

Lena Daumann

Expression, purification and properties of enzymes involved in lanthanide-dependent alcohol oxidation: XoxF4, XoxF5, ExaF/PedH, XoxG4

Ludmila Chistoserdova

Expression, purification and testing of lanthanide-dependent enzymes in Methylorubrum extorquens AM1

Norma Cecilia Martinez-Gomez

Heterologous expression, purification, and characterization of proteins involved in lanthanide utilization

Joseph A. Cotruvo Jr.

Transposon mutagenesis for methylotrophic bacteria using Methylorubrum extorquens AM1 as a model system

Elizabeth Skovran

Metals and Methylotrophy: Global Gene Expression Studies

Marina G. Kalyuzhnaya

Mass spectrometry-based approaches to study lanthanides in the phyllosphere

Julia A. Vorholt

Cultivation techniques to study lanthanide metal interactions in the haloalkaliphilic Type I methanotroph "Methylotuvimicrobium buryatense" 5GB1C

Joseph Douglas Groom Jr.