Main description:

The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select “Courses. ” The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

Back cover:

Regulation of Gene Expression: Molecular Mechanisms presents a comprehensive overview of methods and approaches for characterizing mechanisms of gene regulation. The text is appropriate both as a graduate textbook and a standard laboratory reference and provides the essential groundwork for an advanced understanding of the various mechanisms that may result in altered activity of a specific cell protein.

Each of three sections explores mechanisms of gene regulation and expression, and presents methods and protocols for achieving specific experimental goals. Part I focuses on approaches for studying control of mRNA expression and determining target genes for a given transcription copy. Part II outlines the methods for determining how proteins can regulate each other by mediating synthesis, degradation, protein-protein interactions, and posttranslational modification. Part III explores how gene targeting techniques in mice can provide insight into protein function. This volume provides a clear, concise overview of the protocols and techniques used to examine chemically or disease-mediated alterations in gene expression in mammalian systems.

Contents:

Part I. Gene Expression Control at the mRNA Level

John P. Vanden Heuvel

Chapter 1. Overview

Chapter 2. Messenger RNA Accumulation

Chapter 3. Transcript Profiling

Chapter 4. Transcriptional Regulation of Gene Expression

Chapter 5. Posttranscriptional Processing of Messenger RNA

Study Questions

References

Part II. Regulation of Protein Levels and Transcription Factor Function

Gary H. Perdew

Chapter 1. Overview

Chapter 2. Protein Synthesis and Turnover

Chapter 3. Proteomics

Chapter 4. Determination of Protein-Protein Interactions and the Motifs That Mediate Them

Chapter 5. Posttranslational Modifications

Chapter 6. Dissection of Signaling Pathways

Study Questions

References

Part III. Use of Transgenic and Knockout Mice to Study Gene Regulation

Jeffrey M. Peters

Chapter 1. Overview

Chapter 2. Production of Transgenic Animals

Chapter 3. Knockout Animals

Chapter 4. Isolation of Cells and Cell Lines From Transgenic and Knockout Animals

Study Questions

References

Index