Main description:

The Sample Preparation Techniques for Environmental, Plant, and Animal Samples handbook is a collection of best practices, recipes and theoretical information aimed at anyone who works with any type of molecular biology, proteomics, or metabolomics research involving diffi cult and tough-to-process samples, and thus is exposed to the seemingly unbreakable bottleneck of sample preparation. Th is book is most useful to researchers preparing nucleic acids and proteins from environmental (e.g., soil, marine, and wastewater, feces) and tough microbiological (e.g., spores, yeasts, gram positive bacteria) samples, as well as solid tissue samples from plants and animals. This book is the first comprehensive piece of literature dealing with applications of bead beating technology and other types of mechanical homogenization sample preparation.

Contents:

Contents

Foreword

Ljubisav Rakic

1. Physical and Chemical Properties of Nucleic Acids

Daphne Real

2. Sampling for Biostatistics

Angela Conley and Jason Pfefferkorn

3. Sampling of Microbiological Samples

Christina Stam, Alberto Behar, and Moogega Cooper

4. Techniques for Nucleic Acid Purifications from Plant, Animal, and Microbial Samples

Jordan Ruggieri, Ryan Kemp, Stanislav Forman, and Marc E. Van Eden

5. Magnetic beads based nucleic acids purification for molecular biology applications

Nives Kovacevic

6. Systems for Mechanical Sample Lysis and Homogenization for Molecular Biology Applications

James A. Atwood III

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7. Bead beating offers high performance homogenization for molecular biology downstream processing of tough and difficult samples

Vanessa M. Yu

8. High Performance Bead Beating Based Lysing, Homogenization and Grinding for DNA, RNA and

proteins extraction with FastPrep (R) Systems

Miodrag Micic, Jeffrey D. Whyte, and Veronique Karsten

9. Portable Systems for Sample Lysis and Homogenization

Horacio Kido

10. DNA and RNA extractions from mammalian samples

Shuko Harada

11. Sample preparation for multiplex PCR assays for food and agriculture applications

Rachel Binet and Aparna Tatavarthy

12. Nucleic acid sample preparation from stem cells

Mirjana Pavlovic

13. Nucleic Acids Sample Preparation from Teeth/Dental Remains

Denice Higgins and Jeremy J. Austin

14. Nucleic Acids Sample Preparation for Food Traceability

Leonor Pereira, Sonia Gomes, and Paula Martins-Lopes

15. Nucleic Acids Sample Preparation for Quantitative and Qualitative GMO analysis

Maher Chaouachi

16. Nucleic Acids Samples Preparation from Dairy Products and Milk

Julie Ann Kase and Tina Lusk

17. Extraction of DNA from

Plant Tissue: Review and Protocols

Farshad Tamari and Craig S. Hinkley

18. DNA Extraction from Seeds

Celio Dias Santos Junior, Natalia Melquie Monteiro Teles, Denis Prudencio Luiz, and Thais Ferreira Isabel

19. RNA extractions from difficult to prepare and high starch content seeds

Preeya Puangsomlee Wangsomnuk, Benjawan Ruttwat, Wipavadee Rittitum, Pinich Wangsomnuk, Sanun Jogloy, and Aran Patanothai

20. Nucleic Acid and Protein Sample Preparation from Yeasts

Zhaohua Tang and Gretchen Edwalds-Gilbert

21. Nucleic acids purification from soil and environmental sources

Moogega Cooper and Christina Stam

22. Extraction and Purification of Viral Nucleic Acids from Environmental Samples

Brandon C. Iker, Masaaki Kitajima, and Charles P. Gerba<

23. Preparation of nucleic acids from marine samples: applications to microbial ecology research

Karine Lemarchand, Thomas Pollet, Vincent Lessard, and M. Amine Badri

24. Nucleic Acid Sample Preparation from Feces and Manure

Tim Reuter and Rahat Zaheer

25.

Bead-beating sample preparation for nucleic acids isolation from fecal samples

Jeffrey D. Whyte

26. Methods for extraction and purification of lignin and cellulose from plant tissues

Ksenija Radotic and Miodrag Micic

27. Separation and purification of glycans out of glycoproteins

Olga Gornik, Toma Keser and Gordan Lauc

28. Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional Renilla-prion fusion protein from bacteria

Robert M. Hnasko, Alice V. Lin, Larry H. Stanker, Kumar Bala, and Jeffery A. McGarvey