Main description:

This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.

Contents:

. Generating Genetically Modified Mice: A Decision Guide Ivo J. Huijbers

2. Direct Generation of Conditional Alleles using CRISPR/Cas9 in Mouse Zygotes

Colin Pritchard, Lona J. Kroese, and Ivo J. Huijbers

3. Building Cre Knockin Rat Lines using CRISPR/Cas9

Yuanwu Ma, Lianfeng Zhang, and Xingxu Huang

4. Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases

Eugenia Voziyanova, Rachelle P. Anderson, and Yuri Voziyanov

5. Use of the DICE Dual Integrase Cassette Exchange System

Alfonso P. Farruggio, Mital S. Bhakta, and Michele P. Calos

6. Ligand-controlled Site-specific Recombination in Zebrafish Avinash Chekuru, Veronika Kuscha, Stefan Hans, and Michael Brand

7. Injection-based Delivery of Cell Permeable Peptide-tagged Cre

Wei-Ming Chien, Yonggang Liu, Ana A. Dinca, and Michael T. Ch

in

8. Viral Delivery of GFP-dependent Recombinases to the Mouse Brain

Jonathan C.Y. Tang, Stephanie Rudolph, and Constance L. Cepko

9. Recombinase-mediated Cassette Exchange using Adenoviral Vectors

Andreas F. Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson, and David A. Sorrell

10. Marker Removal in Transgenic Plants using Cre recombinase Delivered with

Potato Virus X

Lilya Kopertekh and Joachim Schiemann

11. Nanoparticle-mediated Recombinase Delivery into Maize

Susana Martin-Ortigosa, Brian G. Trewyn, and Kan Wang

12. Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines

Qi Lu and Zhuo-Hua Pan

13. FLPing Genes On and Off in Drosophila

Bonnie M. Weasn

er, Jinjin Zhu, and Justin P. Kumar

14. Imaging Neural Architecture in Brainbow Samples

Douglas H. Roossien and Dawen Cai

15. Purification and in vitro characterization of Zinc Finger Recombinases

Femi J. Olorunniji, Susan J. Rosser, and W. Marshall Stark

16. Preparing Mate-paired Illumina Libraries using Cre Recombinase

Ze Peng

17. Preparing Fosmid Mate-Paired Libraries Using Cre-LoxP Recombination

Ze Peng, Jeff L. Froula, and Jan-Fang Cheng

18. Using Purified Tyrosine Site-specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways

Wei Liu, Laura R. Tuck, Jon Marles Wright, and Yizhi Cai

19. Multipart DNA Assembly Using Site-specific Recombinases from the Large Serine Integrase Family

Femi J. Olorunniji, Christine Merrick, Susa

n J. Rosser, Margaret C. M. Smith, W. Marshall Stark, and Sean D. Colloms

20. Production of Minicircle DNA Vectors using site Specific Recombinases

Nafiseh Sanei Ata-abadi, Naeimeh Rezaie, Kianoush Dormiani, Mohammad Hossein, and Nasr-Esfahani