Main description:

This volume focuses
on the cell biology and physiology of skeletal muscle regeneration. This Book
is a collection of classic and cutting edge protocols optimized for mice, but
in most cases adaptable to rat or other mammalian models, that will allow an
investigator to develop and implement a research study on skeletal muscle
regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the
progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by
eccentric contraction; volumetric muscle loss; single myofiber isolation and
culture; satellite cell transplantation; muscle clearing for whole mount
immunostaining; luciferase tracking of muscle stem cells; mitochondrial
and mitophagy flux analysis; in vivo assessment of muscle contractility;
force measurements on single isolated myofibers; and analysis of aerobic
respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly
successful Methods in Molecular Biology series format,
chapters include introductions to each respective topic, lists of the necessary
materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and
practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in
skeletal muscle growth, damage, repair, degeneration, and regenerative therapy
in the mouse model system.

Contents:

Part I Injury
Models

1. Eccentric
Contraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Models
to Impair Skeletal Muscle's Capacity to Generate Force
Jarrod A. Call and Dawn
A. Lowe

2. Volumetric
Muscle Loss

Beth E. Pollot and
Benjamin T. Corona

3. Freeze Injury of the Tibialis Anterior
Muscle

Gengyun Le, Dawn A. Lowe, and Michael
Kyba

4. Synergist Ablation
as a Rodent Model to Study Satellite Cell Dynamics in Adult Skeletal Muscle

Tyler J. Kirby, John J. McCarthy,
Charlotte A. Peterson, and Christopher S. Fry

5. Inducing
and Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride

Matthew T.
Tierney and Alessandra Sacco

6. Cardiotoxin Induced Injury and
Skeletal Muscle Regeneration

Glynnis A. Garry, Marie Lue
Antony, and Daniel J. Garry

7. Fibrosis-Inducing Strategies in Regenerating Dystrophic and Normal Skeletal
Muscle

Patrizia
Pessina and Pura Munoz-Canoves

Part II Evaluating Progenitor Cells

8. Isolation, Cryosection, and Immunostaining
of Skeletal Muscle

Huascar P. Ortuste Quiroga,
Katsumasa Goto, and Peter S. Zammit

9. Isolation of Mouse Periocular Tissue
for Histological and Immunostaining Analyses of the Extraocular Muscles and
their Satellite Cells

Pascal
Stuelsatz and Zipora Yablonka-Reuveni

10. Skeletal Muscle Tissue Clearing
for LacZ and Fluorescent Reporters, and Immunofluorescence Staining

Mayank Verma,
Bhavani Sai Rohit Murkonda, Yoko Asakura, and Atsushi Asakura

11. Isolation, Culture, Functional
Assays and Immunofluorescence of Myofiber-Associated Satellite Cells

Thomas O.
Vogler, Katherine E. Gadek, Adam B. Cadwallader, Tiffany L. Elston, and Bradley
B. Olwin

12. Transplantation-Based Quantitative
Assays for Satellite Cell Self-Renewal and Differentiation

Robert W. Arpke
and Michael Kyba

13. Non-Invasive Tracking of Quiescent
and Activated MuSC Engraftment Dynamics In Vivo

Andrew T.V. Ho
and Helen M. Blau

14.
Myogenic Progenitors from Mouse Pluripotent
Stem Cells for Muscle Regeneration

Alessandro
Magli, Tania Incitti, and Rita C.R. Perlingeiro

15. Assaying Human Myogenic Progenitor
Cell Activity by Reconstitution of Muscle Fibers and Satellite Cells in Immunodeficient
Mice

Maura H. Parker

16. Methods for Mitochondria and Mitophagy
Flux Analyses in Stem Cells of Resting and Regenerating Skeletal Muscle

Laura
Garcia-Prat, Marta Martinez-Vicente, and Pura Munoz-Canoves

17. Identification, Isolation, and
Characterization of Mesenchymal Progenitors in Mouse and Human Skeletal Muscle
Akiyoshi Uezumi, Takehiro Kasai, and
Kunihiro Tsuchida

18. FACS Fractionation and Differentiation of Skeletal-Muscle
Resident Multipotent Tie2+ Progenitors
Arpita A. Biswas and David J.
Goldhamer

Part III Muscle Functional Assays

19. In Vitro Assays to Determine
Skeletal Muscle Physiologic Function

Justin E. Sperringer and Robert W. Grange

20. In Vivo Assessment of Muscle
Contractility in Animal Studies

Shama R. Iyer, Ana P. Valencia, Erick
O. Hernandez-Ochoa, and Richard M. Lovering

21. Functional Measurement of
Respiratory Muscle Motor Behaviors Using Transdiaphragmatic Pressure
Sarah M. Greising, Carlos B.
Mantilla, and Gary C. Sieck

22. Assessment of the Contractile Properties
of Permeabilized Skeletal Muscle Fibers
Dennis R.
Claflin, Stuart M. Roche, Jonathan P. Gumucio, Christopher L. Mendias, and
Susan V. Brooks

23. Analysis of Aerobic Respiration
in Intact Skeletal Muscle Tissue by Microplate-Based Respirometry

Jonathan
Shintaku and Denis C. Guttridge