Main description:

Quantitative elucidation of structural, energetic and dynamic aspects of macromolecular interactions is indispensable for understanding the functional activities of biomolecules and their interactions. The optical spectroscopic methods are not confined to small molecules or macromolecules but permit the studies of even the largest biological systems in their full splendor, including the living cell. In, Spectroscopic Methods of Analysis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study properties of individual macromolecules, their complexes, organelles, and cells, using optical spectroscopic techniques. These include methods and approaches for experimental and theoretical analyses of fluorescence properties of the examined systems, single molecule approaches, electronic absorption, and electro-optical analyses of macromolecular interactions, structures, and dynamics. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Spectroscopic Methods of Analysis : Methods and Protocols seeks to aid scientists in the further study of optical spectroscopic methods.

Contents:

1. Fluorescene Lifetime Imaging Comes of Age How to Do It and How to Interpert It

Yi-Chun Chen, Brayan Q. Spring, and Robert M. Clegg

2. Foerster Resonance Energy Transfer and Trapping in Selected Systems Analysis by Monte-Carlo Simulation

P. Bojarski, A. Synak, L. Kulak, S. Rangelowa-Jankowska, A. Kubicki, B. Grobelna

3. Molecular Organization of Polyene Antibiotic Amphotericin B Studied by Means of Fluorescence Technique

Wieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko, Zygmunt Gryczynski, Ignacy Gryczynski

4. Spectroscopic Probes of RNA Structure and Dynamics

Kathleen B Hall

5. Fluorescence Methods to Study DNA Translocation and Unwinding Kinetics by Nucleic Acid Motors

Christopher J. Fischer, Eric J. Tomko, Colin G. Wu, and Timothy M. Lohman

6. Fluorescence Intensity, Anisotropy, and Transient Dynamic Quenching Stopped-Flow Kinetics

Wlodzimierz Bujalowski and Maria J. Jezewska

7. Using Structure - Function Constraints in FRET Studies of Large Macromolecular Complexes.

Wlodzimierz Bujalowski and Maria J. Jezewska

8. Illuminating Allostery in Metal Sensing Transcriptional Regulators

Nicholas E. Grossoehme and David P. Giedroc

9. Fluorescence-based Biosensors

Maria Strianese, Maria Staiano, Giuseppe Ruggiero, Tullio Labella, Claudio Pellecchia, and Sabato D'Auria

10. Metal Enhanced Immunoassays

Ignacy Gryczynski, Rafal Luchowski, Evgenia G. Matveeva, Tanya Shtoyko, Pabak Sarkar, Julian Borejdo, Irina Akopova, Zygmunt Gryczynski

11. Initial Stages of Angiosperm Greening Monitored by Low-Temperature Fluorescence Spectra and Fluorescence Lifetimes

Beata Mysliwa-Kurdziel, Anna Stecka, Kazimierz Strzalka

12. Activation of the Mammalian Cells by using Light-sensitive Ion Channels

Mandy Siu Yu Lung, Paul Pilowsky, Ewa Goldys

13. Detection of Specific Strains of Viable Bacterial Pathogens by using RNA Bead Assays and Flow Cytometry with 2100 Bioanalyzer

Philip Butterworth, Henrique T. Baltar M. C. M., Martin Kratzmeier, Ewa M. Goldys

14. Photosynthetic Antenna Complex LHCII Studied with Novel Fluorescence Techniques

Wieslaw I. Gruszecki, Rafal Luchowski, Wojciech Grudzinski, Zygmunt Gryczynski, Ignacy Gryczynski

15. Analysis of RNA Folding and Ribonucleoprotein Assembly by Single-Molecule Fluorescence Spectroscopy

Goran Pljevaljcic, Rae Robertson-Anderson, Edwin van der Schans and David Millar

16. Single-molecule Force Spectroscopy of Polycystic Kidney Disease Proteins

Liang Ma, Meixiang Xu and Andres F. Oberhauser

17. Single Molecule Detection Approach to Muscle Study: Kinetics of a Single Cross-Bridge During Contraction of Muscle

Julian Borejdo, Danuta Szczesna-Cordary, Priya Muthu, Prasad Metticolla, Rafal Luchowski, Zygmunt Gryczynski, Ignacy Gryczynski

18. Single-Molecule Optical-Trapping Measurements with DNA Anchored to an Array of Gold Nanoposts

D. Hern Paik & Thomas T. Perkins

19. Electro-optical Analysis of Macromolecular Structure and Dynamics

Dietmar Porschke

20. Cryoradiolysis and Cryospectroscopy for Studies of Heme-oxygen Intermediates in Cytochromes P450

I.G.Denisov, Y.V.Grinkova, S.G.Sliga