Main description:

This concise, truncated version of Zini and Agarwal's Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction offers select, research-oriented chapters for a handy, more affordable, state-of-the-art resource. Sperm Chromatin for the Researcher addresses such vital issues as:

- Structure and function of human sperm chromatin

- Biological determinants of sperm chromatin damage

- Laboratory evaluation of sperm chromatin

- Protocols to measure sperm chromatin damage

Ideal for novice and experienced researchers alike, Sperm Chromatin for the Researcher contains all of the need-to-know information about these cutting-edge topics in reproductive medicine.

Contents:

Part I: Human Sperm Chromatin: Structure and Function.- Sperm Chromatin: An Overview.- Sperm Nucleoproteins.- The Relationship Between Chromatin Structure and DNA Damage in Mammalian Spermatozoa.- Chromosome Positioning in Spermatozoa.- Sperm Mitochondrial DNA.- The Sperm Epigenome.- RNA Expression in Male Germ Cells During Spermatogenesis (Male Germ Cell Transcriptome).- Part II: Biological Determinants of Sperm Chromatin Damage.- Spermatogenesis: An Overview.- Role of Oxidative Stress in the Etiology of Sperm DNA Damage.- Abortive Apoptosis and Sperm Chromatin Damage.- Spermiogenesis in Sperm Genetic Integrity.- Part III: Laboratory Evaluation of Sperm Chromatin.- Sperm Chromatin Structure Assay (SCSA (R)): 30 Years of Experience with the SCSA (R).- Sperm Chromatin Dispersion Test: Technical Aspects and Clinical Applications.- Basic and Clinical Aspects of Sperm Chromomycin A3 Assay.- Cytochemical Tests for Sperm Chromatin Maturity.- Acridine Orange Test for Assessment of Human Sperm DNA Integrity.- Laboratory Evaluation of Sperm Chromatin: TUNEL Assay.- Basic and Clinical Aspects of Sperm Comet Assay.- Assays Used in the Study of Sperm Nuclear Proteins.- Sperm Epigenetic Profile.- Clinical Significance of Sperm RNA.- Part IV: Protocols and Integrity Tests.- Sperm Chromatin Structure Assay (SCSA (R)).- Measurement of DNA Damage in Spermatozoa by TUNEL Assay.