Main description:

Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Staphylococcus Epidermidis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Contents:

1. Clinical Characteristics of Infections in Humans due to Staphylococcus epidermidis

Mark E. Rupp

2. Staphylococcus epidermidis Pathogenesis

Michael Otto

3. Identification of Staphylococcus epidermidis in the Clinical Microbiology Laboratory by Molecular Methods

Amity L. Roberts

4. Pulsed Field Gel Electrophoresis of Staphylococcus epidermidis

Richard V. Goering and Paul D. Fey

5. Multilocus Sequence Typing of Staphylococcus epidermidis

Jonathan C. Thomas and D. Ashley Robinson

6. Growth and Preparation of Staphylococcus epidermidis for NMR Metabolomic Analysis

Greg A. Somerville and Robert Powers

7. The Isolation and Analysis of Phenol-Soluble Modulins of Staphylococcus epidermidis

Hwang-Soo Joo and Michael Otto

8. Genetic Manipulation of Staphylococci

Jeffrey L. Bose

9. Isolation of Chromosomal and Plasmid DNA from Staphylococcus epidermidis

Jill K. Lindgren

10. Isolation of Staphylococcus sp. RNA

Tess Eidem

11. Use of Electroporation and Conjugative Mobilization for Genetic Manipulation of Staphylococcus epidermidis

Katherin L. Maliszewski and Austin S. Nuxoll

12. Methods to Generate a Sequence-Defined Transposon Mutant Library in Staphylococcus epidermidis strain 1457

Todd J. Widhelm, Vijay K. Yajjala, Jennifer L. Endres, Paul D. Fey, and Kenneth W. Bayles

13. Examination of Staphylococcus epidermidis biofilms Using Blow Cell Technology

Derek E. Moormeier and Kenneth W. Bayles

14. Rapid Quantitative and Qualitative Analysis of Biofilm Production by Staphylococcus epidermidis under Static Growth Conditions

Elaine M. Waters, Hannah McCarthy, Siobhan Hogan, Marta Zapotoczna, Eoghan O'Neill, and James P. O'Gara

15. Bacteriophage Transduction in Staphylococcus epidermidis

Michael E. Olson and Alexander R. Horswill

16. Mouse Model of Post-Arthroplasty S. epidermidis Joint Infection

Tyler D. Scheer, Kevin E. Lindgren, Carolyn R. Schaeffer, Mark L. Hanke, Curtis W. Hartman, and Tammy Kielian

17. Mouse Model of Staphylococcus Catheter-Associated Biofilm Infection

Cortney E. Heim, Mark L. Hanke, and Tammy L. Kielian

18. Generation of a Central Nervous System Catheter-Associated Infection in Mice with Staphylococcus epidermidis

Jessica N. Snowden

19. Rat Jugular Catheter Model of Biofilm-Mediated Infection

Carolyn R. Schaeffer, Keith M. Woods, and G. M. Longo