Main description:

As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In Stem Cells in Regenerative Medicine: Methods and Protocols, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo. Written in the highly successful Methods in Molecular Biology (TM) series format, these chapters include brief introductions to the topic, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Comprehensive and easy-to-use, Stem Cells in Regenerative Medicine: Methods and Protocols is certain to contribute greatly to the definition of standardized procedures for the manipulation of somatic and embryonic stem cells in research and clinical applications.

Contents:

Preface Contributors Section I: Stem Cell Sources and Manipulation Derivation and Manipulation of Murine Embryonic Stem Cells Alexander Meissner, Sarah Eminli and Rudolf Jaenisch Patterning Mouse and Human Embryonic Stem Cells using Micro-Contact Printing Raheem Peerani, Celine Bauwens, Eugenia Kumacheva, and Peter W. Zandstra Efficient Gene Knockdowns in Human Embryonic Stem Cells using Lentiviral Based RNAi Asmin Tulpule and George Q. Daley Measurement of Cell-Penetrating Peptide-Mediated Transduction of Adult Hematopoietic Stem Cells Aziza P. Manceur and Julie Audet Stem Cell Sources for Regenerative Medicine Ali M. Riazi, Sarah Y. Kwon and William L. Stanford Section II: Regeneration of the Blood System Investigating the Interactions between Hemopoietic Stem Cells and their Niche: Methods for the Analysis of Stem Cell Homing and Distribution within the Marrow following Transplantation Brenda Williams and Susan K. Nilsson Ex Vivo Megakaryocyte Expansion and Platelet Production from Human Cord Blood Stem Cells Valerie Cortin, Nicolas Pineault, and Alain Garnier Ex vivo Generation of Human Red Blood Cells: A New Advance in Stem Cell Engineering Luc Douay and Marie-Catherine Giarratana Section III: Regeneration of the Nervous System Isolation and Manipulation of Mammalian Neural Stem Cells in Vitro Claudio Giachino, Onur Basak and Verdon Taylor Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors Karl J.L. Fernandesand Freda D. Miller Xenotransplantation of Embryonic Stem Cell-Derived Motor Neurons into the Developing Chick Spinal Cord Hynek Wichterle, Mirza Peljto and Stephane Nedelec Transplantation of Neural Stem/Progenitor Cells into Developing and Adult CNS Tong Zheng, Gregory P. Marshall II, K. Amy Chen, and Eric D. Laywell Transplantation of Embryonic Stem Cell-Derived Dopaminergic Neurons in MPTP-Treated Monkeys Jun Takahashi, Yasushi Takagi and Hidemoto Saiki Section IV: Regeneration of the Epidermis Isolation and Culture of Epithelial Stem Cells. Jonathan A. Nowak and Elaine Fuchs Regeneration of Skin and Cornea by Tissue Engineering Danielle Larouche, Claudie Paquet, Julie Fradette, Patrick Carrier, Francois A. Auger and Lucie Germain Section V: Regeneration of the Musculoskeletal System Prospective Isolation of Mesenchymal Stem Cells from Mouse Compact Bone Brenton J. Short, Nathalie Brouard and Paul J. Simmons Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs) Rahul Sarugaser, Jane Ennis, William L. Stanford and John E. Davies Bone Marrow-Derived Mesenchymal Stem Cells: Isolation, Expansion, Characterization, Viral Transduction and Conditioned Media Production Massimiliano Gnecchi and Luis G. Melo Template DNA Strand Co-segregation and Asymmetric Cell Division in Skeletal Muscle Stem Cells Vasily Shinin, Barbara Gayraud-Morel, and Shahragim Tajbakhsh Isolation and Grafting of Single Muscle Fibres Charlotte A. Collins