Main description:

Super-Resolution Microscopy Techniques in the Neurosciences serves as a comprehensive description of current super-resolution techniques, including the physical principles that allowed for their development, some of the most recent neurobiological applications and selected information for the practical use of these technologies. Written for the Neuromethods series, this detailed work contains contributions from experts in the field and provides key implementation advice to ensure successful results in the lab.

Authoritative and cutting-edge, Super-Resolution Microscopy Techniques in the Neurosciences is an ideal guide for researchers aiming to continue increasing the resolution in the imaging capabilities of neuroscientists and thereby changing the perspective in which cellular biology processes are understood at the nanometer scale.

Contents:

1. Light Microscopy and Resolution

Sinem K. Saka

2. Super-Resolution Microscopy: Principles, Techniques, and Applications

Sinem K. Saka

3. Foundations of STED Microscopy

Marcel A. Lauterbach and Christian Eggeling

4. Application of Real-Time STED Imaging to Synaptic Vesicle Motion

Benjamin G. Wilhelm and Dirk Kamin

5. Photoactivated Localization Microscopy for Cellular Imaging

Paulina Achurra, Seamus Holden, Thomas Pengo, and Suliana Manley

6. Data Analysis for Single-Molecule Localization Microscopy

Steve Wolter, Thorge Holm, Sebastian van de Linde, and Markus Sauer

7. Super-Resolution Fluorescence Microscopy Using Structured Illumination

Kai Wicker

8. Application of Three-Dimensional Structured Illumination Microscopy in Cell Biology: Pitfalls and Practical Considerations

Daniel Smeets, Jurgen Neumann, and Lothar Schermelleh

9. Scanning Near-Field Optical Microscopy for Investigations of Bio-Matter

Christiane Hoeppener

10. Atomic Force Microscopy of Living Cells

David Alsteens and Yves F. Dufrene

11. X-Ray Microscopy for Neuroscience: Novel Opportunities by Coherent Optics

Tim Salditt and Tanja Ducic

12. Nonlinear Optics Approaches Towards Sub-Diffraction Resolution in CARS Imaging

Klaus-Jochen Boller, Willem P. Beeker, Carsten Cleff, Kai Kruse, Chris J. Lee, Petra Gross, Herman L. Offerhaus, Carsten Fallnich, and Jennifer L. Herek

13. Photo-Oxidation Microscopy: Bridging the Gap Between Fluorescence and Electron Microscopy

Annette Denker and Silvio O. Rizzoli

14. Requirements for Samples in Super-Resolution Fluorescence Microscopy

Marko Lampe and Wernher Fouquet

15. Probing Biological Samples in High-Resolution Microscopy: Making Sense of Spots

Felipe Opazo