Main description:

T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.

Contents:

Part I. Isolation of T Cells from the Tissues

1. Isolation of T Cells from the Skin

Beate Lorenz and Esther von Stebut

2. Isolation of Leukocytes from Mouse Central Nervous System

Iva Lelios and Melanie Greter

3. Isolation of T Cells from the Gut

Sonja Reissig, Christopher Hackenbruch, and Nadine Hoevelmeyer

4. T Cell Isolation from Mouse Kidneys

Isis Ludwig-Portugall and Christian Kurts

Part II. Analysis of T Cell Function and Phenotypes

5. Intracellular Staining for Cytokines and Transcription Factors

Florian Mair and Vinko Tosevski

6. Surface Capture Staining

Vinko Tosevski and Florian Mair

7. Mass Cytometry Analysis of Human T Cell Phenotype and Function

Evan W. Newell and Lai Li Yun

8. Detection of NF- B Pathway Activation in T Helper Cells

Oliver Gorka, Stefan Wanninger, and Jurgen Ruland

9. Assessing the Suppressive Activity of Foxp3+ Regulatory T Cells

Christian Thomas Mayer and Tim Sparwasser

10. In vitro Generation of Microbe Specific Human Th17 Cells

Julia M. Braun and Christina E. Zielinski

11. In vitro Polarization of T-Helper Cells

Julie Rumble and Benjamin Segal

Part III. In vivo Models of T Cell Function

12. A Method for the Generation of TCR Retrogenic Mice

Elisa Kieback, Ellen Hilgenberg, and Simon Fillatreau

13. Mouse Models of Allergic Airway Disease

Helen Meyer-Martin, Sebastian Reuter, and Christian Taube

14. Induction of Colitis in Mice (T-Cell Transfer Model)

Benno Weigmann

15. Manipulation of T Cell Function Conditional Gene Targeting in T Cells

Anna Sledzinska, Lynsey Fairbairn, and Thorsten Buch

16. Aldara-Induced Psoriasis-Like Skin Inflammation: Isolation and Characterization of Cutaneous Dendritic Cells and Innate Lymphocytes

C.T. Wohn, S. Pantelyushin, J.L. Ober-Bloebaum, and B.E. Clausen

17. Induction of Passive EAE Using Myelin-Reactive CD4+ T Cells

Rhoanne C. McPherson, Helen E. Cambrook, Richard A. O'Connor, and Stephen M. Anderton

18. Experimental Mouse Models of T Cell-Dependent Inflammatory Bowel Disease

George X. Song-Zhao and Kevin J. Maloy

19. Analysis of Chromosomal Aberrations in Murine HCC

Kristian Unger and Mathias Heikenwalder