Main description:

This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

1. Xanthomonas and the TAL effectors: Nature's Molecular Biologist

Frank White

2. TAL Effector DNA-binding Principles and Specificity

Annekatrin Richter, Jana Streubel, and Jens Boch

3. The Development of TALE Nucleases for Biotechnology

David G. Ousterout and Charles A. Gersbach

4. Online Tools for TALEN Design

Adam J. Bogdanove and Nicholas J. Booher

5. Assembly of Customized TAL Effectors through Advanced ULtiMATE System

Junjiao Yang, Shengjie Guo, Pengfei Yuan, and Wensheng Wei

6. Engineering Customized TALENs Using the Platinum Gate TALEN Kit

Tetsushi Sakuma and Takashi Yamamoto

7. Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors

Pratiksha I. Thakore and Charles A. Gersbach

8. A New Approach to Dissect Nuclear Organization; TALE-mediated Genome Visualization (TGV)

Yusuke Miyanari

9. TALEN Induced Translocations in Human Cells

Marion Piganeau, Benjamin Renouf, Hind Ghezraoui, and Erika Brunet

10. Mutagenesis in Newts; Protocol for Iberian Ribbed Newts

Toshinori Hayashi and Takashi Takeuchi

11. Targeted Mutagenesis in Bombyx mori Using TALENs

Yoko Takasu, Toshiki Tamura, Marian Goldsmith, and Michal Zurovec

12. GeneKnockout by Targeted Mutagenesis in a Hemimetabolous Insect, The TwoSpotted Cricket Gryllus bimaculatus, using TALENs

Takahito Watanabe, Sumihare Noji, and Taro Mito

13. Methods for TALEN Evaluation, use, and Mutation Detection in the Mosquito Aedes aegypti

Sanjay Basu, Azadeh Aryan, Mary Etna Haac, Kevin M. Myles, and Zach N. Adelman

14. Methods for TALEN-mediated Genomic Manipulations in Drosophila

Jiyong Liu, Yawen Guo, Changqing Li, Yixu Chen, and Renjie Jiao

15. Targeted Mutagenesis in Zebrafish by TALENs

Peng Huang, An Xiao, Xiangjun Tong, Shuo Lin, and Bo Zhang

16. Mutagenesis in Xenopus and Zebrafish using TALENs

Yun Liu, Hui Zhao, and Christopher H.K. Cheng

17. Genome Editing in Mice using TALE Nucleases

Benedikt Wefers, Christina Brandl, Oskar Ortiz, Wolfgang Wurst, and Ralf Kuhn

18. Genome Editing in Rats using TALE Nucleases

Laurent Tesson, Severine Remy, Severine Menoret, Claire Usal, Reynald Thinard, Chloe Savignard, Anne De Cian, Carine Giovannangeli, Jean-Paul Concordet, and Ignacio Anegon

19 Designer Nuclease-mediated Generation of Knock out THP1 Cells

Tobias Schmidt, Jonathan L. Schmid-Burgk, Thomas S. Ebert, Moritz M. Gaidt, and Veit Hornung