Main description:

The Art & Science of Assisted Reproductive Technology is a comprehensive step by step guide to different types of ART.

The book is divided into fifteen sections, beginning with instructions on setting up an assisted reproductive technology laboratory. The second section is a guide to the evaluation of an infertile couple, including the use of ultrasound and Doppler imaging in infertility, and the effect of the environment on fertility. Further sections include information on third party reproduction, medical and surgical treatments, and gamete donation and manipulation.

The book describes ART techniques that employ molecular genetics, which can maximise the efficiency of ART treatments. Conditions that negatively affect fertility, including sexually transmitted diseases, endometriosis, ectopic pregnancy and immune system conditions are also discussed in detail.

The Art & Science of Assisted Reproductive Technology covers all aspects of ART, from laboratory set up, embryo transfer and implantation, to cryopreservation (freezing) of eggs, sperm and embryos. Enhanced by nearly 300 full colour images, illustrations and tables, this book an essential resource for gynaecologists and infertility specialists.

Key Points

Comprehensive step by step guide to all aspects of assisted reproductive technology
Includes guide to set up of an ART laboratory
Information on ART in special conditions, third party ART and complications in ART
287 full colour images, illustrations and tables

Contents:

Section 1 Setting-up an ART Laboratory

How to Set-up an ART Laboratory
Quality Control in an ART Laboratory
Quality Care Management in ART Clinic

Section 2 How to Investigate an Infertile Couple

Evaluation of the Male Partner
Investigation of Infertile Female
Ultrasound and Doppler in Infertility
Effect of Environment on Fertility

Section 3 Endoscopy in Infertility

Fertility Enhancing Laparoscopic Surgery
Fertility Enhancing Hysteroscopic Surgery
Pilot Hysteroscopy before ART

Section 4 Endocrinology and Stimulation Protocols

Genetic Markers of Ovarian Response
Physiology of Follicular Phase
Progesterone in Follicular Phase during Controlled Ovarian Stimulation
Therapeutic Window for Luteinizing Hormone in Controlled Ovarian Stimulation
Stimulation Protocols for ART
Stimulation Protocols for Polycystic Ovarian Syndrome in ART
GnRH Agonists and Antagonists in Assisted Reproduction
Monitoring Ovarian Stimulation
Ovulation Trigger

Section 5 Gamete Collection and Preparation

Oocyte Retrieval
Oocyte Evaluation and Preparation
Semen Analysis and Evaluation of Sperm
Semen Collection and Preparation

Section 6 Micromanipulation

Micromanipulation: Instrument, Technique and ICSI Procedure

Section 7 Enhancing Sperm, Oocyte, Embryo Quality

Polarized Light Microscopy
IMSI in Daily Laboratory Practice: Lights and Shadows
Role of Preimplantation Genetic Diagnosis in ART
Assisted Hatching of Human Embryos

Section 8 Culture of Embryos and Transfer

Culture and Evaluation of Embryos: From Fertilization to Transfer
Proteomics and Metabolomics in ART
Time-lapse Microscopy
Embryo Transfer: Catheters and Techniques

Section 9 Implantation

Endometrial Receptivity
Embryo Endometrial Dialogue
Physiology of Implantation in Natural Cycle versus Stimulated Cycle
Recurrent Implantation Failure: Strategies and Intervention
Implantation and the Endometrium in Uterine Fibroid
Implantation in Endometriosis

Section 10 Cryopreservation

Oocyte Cryopreservation
Embryo Cryopreservation
Cryopreservation of Ovarian Tissue
In Vitro Maturation of Oocytes
Semen Banking
Fertility Preservation in Cancer (Male and Female)

Section 11 Luteal Phase in ART

Luteal Phase Support in ART

Section 12 ART in Special Conditions

Endometriosis and ART
Myomas in Infertility
Genital Tuberculosis and ART
Poor Ovarian Response
ART in Polycystic Ovary Syndrome

Section 13 Third Party ART

Gamete Donation
Embryo Donation
Surrogacy

Section 14 Pregnancy and ART

First Trimester Screening in Pregnancies Conceived with ART
Obstetric Outcome Following ART
ART to Antenatal Care

Section 15 Complications in ART

Ovarian Hyperstimulation Syndrome
Ovarian Cancer and ART
Ethical and Stress Issues in ART
Index