Main description:

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

Contents:

Section I: Measuring and Modifying the UPR

Methods for investigating the UPR in filamentous fungi Thomas Guillemette, Arthur F.J. Ram, Neuza D.S.P. Carvalho, Aymeric Joubert, Philippe Simoneau, David B. Archer
Assays for detecting the unfolded protein response Karen Cawley, Shane Deegan, Afshin Samali, Sanjeev Gupta
Analysis of the role of nerve growth factor in promoting cell survival during endoplasmic reticulum stress in PC12 cells Koji Shimoke , Harue Sasaya, and Toshihiko Ikeuchi
Measuring ER stress and the unfolded protein response using mammalian tissue culture system Christine M. Oslowski and Fumihiko Urano
Real-Time Monitoring of Er Stress in Living Cells And Animals Using Estrap Assay Masanori Kitamura and Nobuhiko Hiramatsu
HIV protease inhibitors induce endoplasmic reticulum stress and disrupt barrier integrity in intestinal epithelial cells Huiping Zhou
Dexamethasone induction of a heat-stress response Jose A. Guerrero, Vicente Vicente, Javier Corral
Detecting and Quantitating Physiological Endoplasmic Reticulum Stress Ling Qi, Liu Yang and Hui Chen Section II: Molecular Components of the UPR
PI 3-Kinase Regulatory Subunits as Regulators of the Unfolded Protein Response Jonathon N. Winnay and C. Ronald Kahn
The Emerging Role of Histone Deacetylases (HDACs) in UPR regulation Soumen Kahali, Bhaswati Sarcar & Prakash Chinnaiyan
Immunohistochemical detection of Activating Transcription Factor 3, a hub of the cellular adaptive-response network Tsonwin Hai, Swati Jalgaonkar, Christopher C. Wolford, and Xin Yin
Experimental approaches for elucidation of stress-sensing mechanisms of the Ire1 family proteins Daisuke Oikawa and Yukio Kimata
Measurement and Modification of the Expression Level of the Chaperone Protein and Signaling Regulator GRP78/BiP in Mammalian Cells Wan-Ting Chen and Amy S. Lee
The Endoplasmic Reticulum associated degradation and disulfide reductase ERdj5 Ryo Ushioda and Kazuhiro Nagata
Structural Insight into the Protective Role of P58(IPK) during Unfolded Protein Response Jiahui Tao, Bingdong Sha
Principles of IRE1 modulation using chemical tools Kenneth P. K. Lee and Frank Sicheri
Methods to study stromal-cell derived factor 2 in the context of ER stress and the unfolded protein response in Arabidopsis thaliana Andrea Schott & Sabine Strahl
Nitrosative stress-induced S-glutathionylation of protein disulfide isomerase Joachim D. Uys, Ying Xiong, and Danyelle M. Townsend
Methods for Analyzing eIF2 Kinases and Translational Control in the Unfolded Protein Response Brian F. Teske, Thomas D. Baird, and Ronald C. Wek