Main description:

Therapeutic Peptides: Methods and Protocols features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage. With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and dependable, Therapeutic Peptides: Methods and Protocols is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates.

Contents:

1. Design of Phage-Displayed Cystine-Stabilized Mini-Protein Libraries for Proteinaceous Binder Engineering

Hung-Ju Chang and An-Suei Yang

2. Construction of a Filamentous Phage Display Peptide Library

Annette Fagerlund, Astrid Hilde Myrset, and Mari Ann Kulseth

3. Engineering Bio-Active Peptide-Based Therapeutic Molecules

Jong Sang Ryu, A. Yeon Cho, Sang Won Seo, and Hosung Min

4. T7 Lytic Phage-Displayed Peptide Libraries: Construction and Diversity Characterization

Lauren R.H. Krumpe and Toshiyuki Mori

5. Affinity Selection Using Filamentous Phage Display

Mari Ann Kulseth, Annette Fagerlund, and Astrid Hilde Myrset

6. Bioprospecting Open Reading Frames for Peptide Effectors

Ling Xiong and Charles Scott

7. Identification of Ideal Peptides for Heterovalent Ligands

Ajay Shrivastava, Matthew von Wronski, Michael F. Tweedle, and Adrian D. Nunn

8. Substrate Phage Display for Protease Substrate Sequence Characterization: Bovine Factor Xa as a Model System

Hung-Ju Hsu and An-Suei Yang

9. Engineering Peptide Therapeutics Using MIMETIBODY (TM) Technology

Kristen Picha, Chichi Huang, Peter Bugelski, and Karyn O'Neil

10. Evaluation of Peptides as Protease Inhibitors and Stimulators

Hannu Koistinen, Can Hekim, Ping Wu, Ale Narvanen, and Ulf-Hakan Stenman

11. Assessment of Antimicrobial (Host Defense) Peptides as Anti-Cancer Agents

Susan Douglas, David Hoskin, and Ashley Hilchie

12. Peptide Labelling Strategies for Imaging Agents

Tuulia Huhtala, Janne Weisell, Jussi Rytkoenen, and Ale Narvanen

13. Peptide Optimization and Conjugation Strategies in the Development of Molecularly Targeted Magnetic Resonance Imaging Contrast Agents

Andrew F. Kolodziej, Zhaoda Zhang, Kirsten Overoye-Chan, Vincent Jacques, and Peter Caravan

14. Evaluation of Prenylated Peptides for Use in Cellular Imaging and Biochemical Analysis

Joshua D. Ochocki, Urule Igbavboa, W. Gibson Wood, Elizabeth V. Wattenberg, and Mark D. Distefano

15. Ultraviolet Absorption Spectroscopy of Peptides

Mangala R. Liyanage, Kunal Bakshi, David B. Volkin, and C. Russell Middaugh

16. Fluorescence Spectroscopy of Peptides

Mangala R. Liyanage, Kunal Bakshi, David B. Volkin, and C. Russell Middaugh

17. Circular Dichroism of Peptides

Kunal Bakshi, Mangala R. Liyanage, David B. Volkin, and C. Russell Middaugh

18. Fourier Transform Infrared Spectroscopy of Peptides

Kunal Bakshi, Mangala R. Liyanage, David B. Volkin, and C. Russell Middaugh