Main description:

The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology (TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and state of the art, Transgenic Mouse Methods and Protocols, second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.

Contents:

Table of Contents

Preface

Contributors

1. Introduction: Strategies for developing genetically modified mice
Marten H. Hofker

2. Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst Injection
Jan Willem Vonken

3. Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos
Jerome Artus and Anna-Katerina Hadjantonakis

4. Cryopreservation of mouse spermatozoa and In vitro fertilization
Naomi Nakagata

5. Autopsy and histological analysis of the transgenic mouse
Marion J.J. Gijbels and Menno P.J. de Winther

6. Transgene design
Bart van de Sluis and Jan Willem Vonken

7. Inducible Transgenic Mouse Models
Thomas L. Saunders

8. Lentiviral transgenesis
Terunaga Nakagawa and Casper C. Hoogenraad

9. Transgenesis in Mouse Embryonic Stem Cells
Janine van Ree, Wei Zhou, Ming Li and Jan van Deursen

10. Engineering BAC Reporter Gene Constructs for Mouse Transgenesis
Yu Fu and Peter Maye

11. Targeting Vector Construction Through Recombineering
Liviu A. Malureanu

12. Generating Conditional Knockout Mice
Roland H. Friedel, Wolfgang Wurst, Benedikt Wefers
and Ralf Kuhn

13. Hypomorphic mice
Daren J. Baker

14. MICER Targeting Vectors for Manipulating the Mouse Genome
Chunhong Liu, Paul F. Szurek and Y. Eugene Yu

15. Knockin approaches
Anton J.M. Roebroek, Philip L.S.M. Gordts and Sara Reekmans

16. Generation of a series of knockin alleles using RMCE in ES cells
Anton J.M. Roebroek, Philip L.S.M. Gordts and Sara Reekmans

17. Selection of targeted mutants from a library of randomly
mutagenized ES cells
Kyoji Horie, George Gaitanaris, and Alexander Gragerov

18. Generation of genetically modified rodents using random ENU mutagenesis
Ruben van Boxtel and Edwin Cuppen

19. Bone marrow transplantations to study gene function in hematopoietic cells
Menno P.J. de Winther and Peter Heeringa

20. Adenovirus Mediated Gene Transfer
Ko Willems van Dijk, Kyriakos E. Kypreos, Frits J. Fallaux and Jurre Hageman