Main description:

The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.

Contents:

Type 1 Diabetes: Prediction And Prevention: Current Perspectives.- The HLA.- Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants.- Islet Autoantibody Analysis: Radioimmunoassays.- Islet Autoantibody Analysis: Non-Radioactive Assays - Electrochemiluminescence.- Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function in Type 1 Diabetes.- Histology of the Type 1 Diabetes Pancreas.- Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.- Tracking Immunological Responses of Islet Antigen-Specific T Cells In The Non Obese Diabetic (NOD) Mouse Model Of Type 1 Diabetes.- Adoptive Transfer of Autoimmune Diabetes using Immunodeficient Non Obese Diabetic (NOD) Mice.- Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes.- FISH with Concomitant Immunofluorescence.- Laser Capture Microdissection: Single Pancreatic Cell DNA Profiling.- The Gut Microbiome in the NOD Mouse.- Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes.