Main description:

Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology (TM) series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.

Contents:

1 Using Fluorescent Proteins to Study Poxvirus Morphogenesis
Brian M. Ward

2 Application of Reporter Gene Imaging for Studying Human Embryonic Stem Cells in Living Subjects
Kazim H. Narsinh, Feng Cao, and Joseph C. Wu

3 Use of a GFP-PML-expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection
Tomonori Ueno and Kiyoko Ogawa-Goto

4 Dual-Color Imaging of Tumor Angiogenesis
Robert M. Hoffman

5 Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous System
Alan R. Harvey, Erich Ehlert, Joris de Wit, Eleanor S. Drummond, Margaret A. Pollett, Marc Ruitenberg, Giles W. Plant, Joost Verhaagen, and Christiaan Levelt

6 Expression of EGFP by Adenovirus-mediated Gene Transfer in the Central Nervous System
Ryohei Tomioka

7 Adenovirus-mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes
Andreas Rinne, Christoph Littwitz, Kirsten Bender, Marie-Cecile Kienitz, and Lutz Pott

8 Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry
Michael Grusch, Annemarie Losert, Andreas Lackner, Alev Deli, Irene Herbacek, and Klaus Holzmann

9 Production of Multicistronic HIV-1 Based Lentiviral Vectors
Nachimuthu Chinnasamy, James Shaffer, and Dhanalakshmi Chinnasamy

10 Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps
Claude Bagnis, Pascal Bailly, and Sylvie Chapel-Fernandes

11 Use of a Macrophage-tropic GFP-tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs
Amanda M. Brown

12 GFP-Lentiviral Vectors Targeting for NeuroAIDS
Yuanan Lu

13 Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery
Aaron M. Jasnow, Donald G. Rainnie, Kimberly A. Maguschak, Jasmeer P. Chhatwal, and Kerry J. Ressler

14 Selective Protein Expression Within the CNS Using Hybrid Lentivirus
Fredrik Gussing, Ingrid van Marion, Igor Ralets, and Cecilia Lundberg

15 Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein
Bruce W. Banfield and Gregory A. Bird

16 The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication
Travis J. Taylor and David M. Knipe

17 Modified Bovine Herpesvirus 1 for Protein Secretion
Gunther M. Keil

18 Identification of Viral Peptide Fragments for Vaccine Development
Zhanglin Lin, Shuang Li, and Yong Chen

19 Use of GFP to Investigate Expression of Plant-derived Vaccines
Hong-Ye Li and Mee-Len Chye

20 Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions
Sophie Haupt, Angelika Ziegler, Graham Cowan, and Lesley Torrance

21 Sendai Virus for Cancer Immunotherapy
Yasuji Ueda, Mamoru Hasegawa, and Yoshikazu Yonemitsu
22 Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells
Qigui Yu, Ningjie Hu, and Mario Ostrowski

23 Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity
Misako Yoneda, Kentaro Fujita, Hiroki Sato, and Chieko Kai

24 Baculovirus-Mediated Gene Transfer into Mesenchymal Stem Cells
Chien-Tai Tsai, Ching-Kuang Chuang, and Yu-Chen Hu