Main description:

Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.

Contents:

Part I: Molecular Tools and Technology for Yeast Engineering

1. An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae

Verena Siewers

2. Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae

Georg Hubmann, Johan M. Thevelein, and Elke Nevoigt

3. Tools for Genetic Engineering of the Yeast Hansenula polymorpha

Ruchi Saraya, Loknath Gidijala, Marten Veenhuis, and Ida J. van der Klei

4. Molecular Tools and Protocols for Engineering the Acid Tolerant Yeast Zygosaccharomyces bailii as a Potential Cell Factory

Paola Branduardi, Laura Dato, and Danilo Porro

5. Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris

Michael Felber, Harald Pichler, and Claudia Ruth

6. Methods for Efficient High Throughput Screening of Protein Expression in Recombinant Pichia pastoris Strains

Andrea Camattari, Katrin Weinhandl, and Rama K. Gudiminchi

7. Synthetic RNA Switches for Yeast Metabolic Engineering: Screening Recombinant Enzyme Libraries

Joshua K. Michener and Christina D. Smolke

8. Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology

Kouichi Kuroda and Mitsuyoshi Ueda

9. Genetic Engineering of Industrial Saccharomyces cerevisiae Strains Using a Selection/Counter-Selection Approach

Dariusz R. Kutyna, Antonio G. Cordente, and Cristian Varela

10. Evolutionary Engineering of Yeast

Ceren Alkim, Burcu Turanli-Yildiz, and Z. Petek Cakar

11. Determination of a Dynamic Feeding Strategy for Recombinant Pichia pastoris Strains

Oliver Spadiut, Christian Dietzsch, and Christoph Herwig

Part II: Tools and Technologies for Investigation and Determination of Yeast Metabolic Features

12. Yeast Metabolomics: Sample Preparation for a GC/MS-Based Analysis

Sonia Carneiro, Rui Pereira, and Isabel Rocha

13. 13C-Based Metabolic Flux Analysis in Yeast: The Pichia pastoris Case

Pau Ferrer and Joan Albiol

14. Pathway Activity Profiling (PAPi): A Tool for Metabolic Pathway Analysis

Raphael B.M. Aggio

15. QTL Mapping by Pooled-Segregant Whole Genome Sequencing in Yeast

Thiago M. Pais, Maria R. Foulquie-Moreno, and Johan M. Thevelein

Part III: Metabolic Models for Yeast Metabolic Engineering

16. Genome Scale Metabolic Models of Yeast, Methods for Their Reconstruction, and Other Applications

Sergio Bordel

17. Model-Guided Identification of Gene Deletion Targets for Metabolic Engineering in Saccharomyces cerevisiae

Ana Rita Brochado and Kiran Raosaheb Patil

Part IV: Patenting and Regulations

18. Patents: A Tool to Bring Innovation from the Lab Bench to the Marketplace

Z. Ying Li and Wolfram Meyer