Main description:

This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Zygotic Genome Activation: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA.

Contents:

Preface...
Table of Contents...
Contributing Authors...
1. Clearance of Maternal RNAs: Not a Mummy's Embryo Anymore
Antonio Marco

2. Link of Zygotic Genome Activation and Cell Cycle Control
Boyang Liu and Joerg Grosshans

3. Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA during Embryogenesis
Alessandro Rosa and Ali H. Brivanlou

4. Gene Expression Analysis of Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR
Kyeoung-Hwa Kim, Su-Yeon Lee, and Kyung-Ah Lee

5. Detection of miRNA in Mammalian Oocytes and Embryos
Malavika K. Adur, Benjamin J. Hale, and Jason W. Ross

6. Detection of Bidirectional Promoter-Derived IncRNAs from Small-Scale Samples using Pre-Amplification-Free Directional RNA-Seq Method
Nobuhiko Hamazaki, Kinichi Nakashima, Katsuhiko Hayashi, and Takuya Imamura

7. Detection and Characterization of Small Non-Coding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation
Jesus Garcia-Lopez, Eduardo Larriba, and Jesus del Mazo

8. Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos
Patricia Heyn and Karla M. Meugebauer

9. RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos
Noemie Ranisavljevic, Ikuhiro Okamoto, Edith Heard, and Katia Ancelin

10. Detection of RNA Polymerase II in Mice Embryos during Zygotic Genome Activation using Immunocytochemistry
Irina O. Bogolyubova and Dmitry S. Bogolyubov

11. Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo
Yan Li and Christopher O'Neill

12. Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos
Ka Yi Ling, Lih Fenh Cheow, Stephen R. Quake, William F. Burkholder, and Daniel M. Messerschmidt

13. Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation
Kyungjun Uh and Kiho Lee

14. Targeted Gene Knockdown in Early Embryos using siRNA
Lu Zhang and Zoltan Machaty

15. Generating Mouse Models using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput
Wenbo Wang, Yingfan Zhang, and Haoyi Wang

16. CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine
Junghyun Ryu and Kiho Lee

17. Potential Involvement of SCF-Complex in Zygotic Genome Activation during Early Bovine Embryo Development
Veronika Benesova, Veronika Kinterova, Jiri Kanka, and Tereza Toralova

18. Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes
Keisuke Aoshima, Takashi Kimura, and Yuki Okada