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MORE ABOUT THIS BOOK
Main description:
This book summarizes the most advanced technical aspects covering all steps for a thorough application of microarrays to inflammation topics - from sample generation to data analysis. In addition selected examples of successful applications of microarrays in major fields of inflammation research are presented. The book will help a researcher or clinician to plan, perform and analyze or to critically review microarray experiments related to inflammation research.
Contents:
Microarray and inflammation: An introduction.- Microarray and inflammation: An introduction.- Methods and protocols for the generation of gene expression profiles in inflammation research.- Whole blood.- Separation of whole blood cells and its impact on gene expression.- Skin and skin models.- Adipose tissue.- Samples isolated by flow cytometry.- Magnetic cell sorting.- T7 based amplification protocols.- Amplification of cDNA from single or rare cells by global PCR (exponential amplification).- Selected applications of microarrays in inflammation research.- Gene expression patterns in asthma.- Gene expression profiling in neurological and neuroinflammatory diseases.- Aspects of gene expression in B cell lymphomas.- The genes behind rheumatology.- miRNA patterns in hematopoietic malignancies.- Keep it simple: microarray cross-platform comparison without statistics.- Microarrays in drug development: regulatory perspective.- Outlook.- Complementary microarray technologies.
PRODUCT DETAILS
Publisher: Springer (Birkhauser Verlag AG)
Publication date: October, 2008
Pages: 243
Weight: 585g
Availability: Available
Subcategories: Diseases and Disorders, Genetics, Haematology, Immunology, Pharmacology
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