Main description:

The groundbreaking new book that covers platelets, their role,and their study through proteomics research

Platelets are defined as small anucleate cells that circulate inthe blood and play a fundamental role in haemostasis andthrombosis. From a pharmacological perspective, unwanted plateletactivation forms blood clots and promotes cardiovasculardisease–the leading cause of death in the Western world.

Platelet Proteomics focuses on the cutting–edge sciencein the emerging field of platelet proteomics research–a vitalanalytical method for the study of platelet activity. Written by avariety of contributions at the forefront of platelet research,this invaluable reference covers the recent developments inanalyzing the proteome of basal and activating platelets inproviding opportunities for advancing the discovery of new proteinsthat could eventually become drug targets for platelet–basedillness. Features of this book include:

• 
  
  Coverage of non–gel based separation methods (MudPit),analysis of the general platelet proteome and signaling cascades bygel–based separation methods (2–DE), and sub–proteome analyses(secretome/releasate, membrane proteins, organelles)
  


• 
  
  A focus on the main research done on platelet proteomics, suchas general proteome mapping, mapping signaling pathways, secretomeand membrane proteomics, studies integrating other –omics, and application to platelet–relateddiseases
  


• 
  
  An examination of platelet–specific problems in a widelyapplicable fashion–the signaling proteome chapter serves as a modelfor investigation in many other cell types.
  

Covering key principles as well as the latest applications inproteome analyses, Platelet Proteomics makes a significantcontribution to an area of study with exciting potential. Thisinaugural foray into platelet exploration is a blueprint for thefuture and is expansive enough in scope to reach across manyscientific disciplines. 

Back cover:

The groundbreaking new book that covers platelets, their role,and their study through proteomics research

Platelets are defined as small anucleate cells that circulate inthe blood and play a fundamental role in haemostasis andthrombosis. From a pharmacological perspective, unwanted plateletactivation forms blood clots and promotes cardiovasculardisease–the leading cause of death in the Western world.

Platelet Proteomics focuses on the cutting–edge sciencein the emerging field of platelet proteomics research–a vitalanalytical method for the study of platelet activity. Written by avariety of contributions at the forefront of platelet research,this invaluable reference covers the recent developments inanalyzing the proteome of basal and activating platelets inproviding opportunities for advancing the discovery of new proteinsthat could eventually become drug targets for platelet–basedillness. Features of this book include:

• 
  
  Coverage of non–gel based separation methods (MudPit),analysis of the general platelet proteome and signaling cascades bygel–based separation methods (2–DE), and sub–proteome analyses(secretome/releasate, membrane proteins, organelles)
  


• 
  
  A focus on the main research done on platelet proteomics, suchas general proteome mapping, mapping signaling pathways, secretomeand membrane proteomics, studies integrating other –omics, and application to platelet–relateddiseases
  


• 
  
  An examination of platelet–specific problems in a widelyapplicable fashion–the signaling proteome chapter serves as a modelfor investigation in many other cell types.
  

Covering key principles as well as the latest applications inproteome analyses, Platelet Proteomics makes a significantcontribution to an area of study with exciting potential. Thisinaugural foray into platelet exploration is a blueprint for thefuture and is expansive enough in scope to reach across manyscientific disciplines. 

Contents:

Contributors.

Foreword.

Preface.

Acronyms.

PART I GENERAL OVERVIEW: PLATELETS, SAMPLE PREPARATION, ANDMASS SPECTROMETRY–BASED PROTEOMICS.

1 Platelets and Their Role in Thrombotic and CardiovascularDisease: The Impact of Proteomic Analysis (Ronald G. Stanley,Katherine L. Tucker, Natasha E. Barrett, and Jonathan M.Gibbins).

2 Mass–Spectrometry–Based Proteomics: General Overview andPosttranslational Modification Analysis in the Context of PlateletResearch (Julia Maria Burkhart and Albert Sickmann).

3 Sample Preparation Variables in Platelet Proteomics forBiomarker Research (Maria Zellner and Rudolf Oehler).

PART II ANALYSIS OF THE PLATELET PROTEOME: GLOBALAPPROACHES AND SUBPROTEOMES.

4 Two–Dimensional Gel Electrophoresis: Basic Principles andApplication to Platelet Signaling Studies (AngelGarcia).

5 The Platelet Membrane Proteome (Yotis A. Senis).

6 Proteomics of Platelet Granules, Organelles, and Releasate(James P. McRedmond).

7 The Platelet Microparticle Proteome (David M.Smalley).

8 N–Terminal Combined Fractional Diagonal Chromatographic(COFRADIC) Analysis of the Human Platelet Proteome (FrancisImpens, Kenny Helsens, Niklaas Colaert, Lennart Martens, JoelVandekerckhove, and Kris Gevaert).

PART III INTEGRATED "OMICS" AND APPLICATIONTO DISEASE.

9 Serial Analysis of Gene Expression (SAGE) For Studying thePlatelet and Megakaryocyte Transcriptome (Michael G.Tomlinson).

10 The Application of Microarray Analysis and Its Integrationwith Proteomics for Study of Platelet–Associated Disorders(Dmitri V. Gnatenko and Wadie F. Bahou).

11 Platelet Functional Genomics (Isabelle I. Salles, Marie N.O Connor, Daphne C. Thijssen–Timmer, Katleen Broos, and HansDeckmyn).

12 Systems Biology to Study Platelet–Related Bleeding Disorders(Jan–Willem N. Akkerman and Bernard De Bono).

13 Platelet Proteomics in Transfusion Medicine (ThomasThiele, Leif Steil, Uwe V¨olker, and AndreasGreinacher).

14 Cardiovascular Proteomics (Fernando Vivanco, Fernando dela Cuesta, Maria G. Barderas, Irene Zubiri, and GloriaA´lvarez–Llamas).

Index.