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MORE ABOUT THIS BOOK
Main description:
Interdisciplinarity is more often invoked than practised. This is hardly surprising, considering the daunting vastness of modern biology. To reach a satisfactory understanding of a complex biological system, a wide spectrum of conceptual and experimental tools must be applied at different levels, from the molecular to the cellular, tissue and organismic. We believe the multifaceted regulatory interplay between integrin receptors and ion channels offers a rich and challenging field for researchers seeking broad biological perspectives. By mediating cell adhesion to the extracellular matrix, integrins regulate many developmental processes in the widest sense (from cell choice between differentiation and proliferation, to tissue remodeling and organogenesis). Rapidly growing evidence shows that frequent communication takes place between cell adhesion receptors and channel proteins. This may occur through formation of multiprotein membrane complexes that regulate ion fluxes as well as a variety of intracellular signaling pathways. In other
cases, cross talk is more indirect and mediated by cellular messengers such as G proteins. These interactions are reciprocal, in that ion channel stimulation often controls integrin activation or expression. From a functional standpoint, studying the interplay between integrin receptors and ion channels clarifies how the extracellular matrix regulates processes as disparate as muscle excitability, synaptic plasticity and lymphocyte activation, just to mention a few. The derangement of these processes has many implications for pathogenesis processes, in particular for tumor invasiveness and some cardiovascular and neurologic diseases. This book provides a general introduction to the problems and methods of this blossoming field.
Contents:
1. Integrin Structure and Functional Relation with Ion Channels
Annarosa Arcangeli and Andrea Becchetti
Abstract
Introduction
Fundamentals of Integrin Structure
Physiological and Pathological Implications: an Outline of Current Trends
Conclusion
2. Introduction to Ion Channels
Chiara Di Resta and Andrea Becchetti
Abstract
Introduction
The Physiology of Ion Channels
Ion Channel Types Involved in Integrin?Mediated Signaling
Conclusion
3. Biochemical Methods to Study the Interactions Between Integrins and Ion Channels
Olivia Crociani
Abstract
Introduction
Yeast Two?Hybrid Screening
Affinity?Based Screening: IP Assays
Pull?Down Assay
Photoaffinity Labeling Techniques for Studying Transient Protein?Protein
Interaction
Far Western Blot Analysis (Far WB)
High?Throughput Protein?Protein Interaction Analysis, Followed by V alidation of Candidate Interactors through Different Experimental Approaches
Past and Future Application of Biochemical Approaches to Characterize
the Interactions between Integrins and Ion Channels
4. Optical Methods in the Study of Protein?Protein Interactions
Alessio Masi, Riccardo Cicchi, Adolfo Carloni, Francesco Saverio Pavone
and Annarosa Arcangeli
Abstract
Introduction
Foerster Resonance Energy Transfer: The "Molecular Ruler"
Intensity Versus Lifetime: Two Ways to Measure FRET
Total Internal Reflection Fluorescence Microscopy (TIRFM) and Imaging
of Membrane Proteins
Antibody?Based Versus Fusion Protein?Based FRET: Principles
Antibody?Based Versus Fusion Protein?Based FRET: Advantages and Disadvantages
Application of Optical Methods to the Study of Integrins and Ion Channels
Conclusion
5. Integrins and Signal Transduction
Sara Cabodi, Paola Di Stefano, Maria del Pilar Camacho Leal, Agata Tinnirello, Brigitte Bisaro, Virginia Morello, Laura Damiano, Simona Aramu,
Daniele Repetto, Giusy Tornillo and Paola Defilippi
Abstract
Overview of Integrin Structure
The SFK?Fak?p130Cas Signaling
Conclusion
6. Physical andFunctional Interaction between Integrins and hERG1 Channels in Cancer Cells
Serena Pillozzi and Annarosa Arcangeli
Abstract
Introduction
hERG1 Channels in Cancer Cells
Effects of Integrin Activation on hERG1 Channels
Integrins and hERG1 Channels form a Macromolecular Complex
Effects of hERG1 Activation on Integrin Function and Signaling
Conclusion
7. Coordinated Regulation of Va scular Ca2+ and K+ Channels by Integrin Signaling
Peichun Gui, Jun?Tzu Chao, Xin Wu, Yan Yang, George E. Davis and Michael J. Davis
Abstract
Introduction
Regulation of L?Type Calcium Channels by Integrin Activation
Regulation of Ca2+?Dependent Potassium Channels by Integrin Activation
Conclusion: The Physiological Relevance of Coordinated Regulation of CaL
and BK Channels by Integrins
8. Adhesion?Dependent Modulation of Macrophage K+ Channels
Margaret Colden?Stanfield
Abstract
Introduction
Inwardly Rectifying K+ (Kir) Currents
Physiologic and Pathophysiologic Roles of Macrophage Kir Channels
Delayed, Outwardly Rectifying K+ (Kdr) Currents
Physiologic and Pathophysiologic Roles of Macrophage Kdr Channels
Conclusion
9. Integrin Receptors and Ligand?Gated Channels
Raffaella Morini and Andrea Becchetti
Abstract
Introduction
The Functional Significance of Integrins in the Adult Brain
Integrins and Nicotinic Acetylcholine Receptors: Not Only the Neuromuscular Junction
Conclusion
10. Integrins and Ion Channels in Cell Migration: Implications for Neuronal Development, Wound Healing and Metastatic Spread
Andrea Becchetti and Annarosa Arcangeli
Abstract
Introduction
The Role of Integrins in Cell Migration
The Role of Ion Channels and Crosstalk with Integrins in Cell Migration
Ca2+ Signaling and the Axonal Growth Cone
Ion Channels as Adhesion Molecules
The Cellular Environment and the Metastatic Process
Invasiveness of Glial Tumors and Ion Channels
Conclusion
Index
PRODUCT DETAILS
Publisher: Springer (Springer-Verlag New York Inc.)
Publication date: April, 2010
Pages: 144
Weight: 349g
Availability: Available
Subcategories: General Issues
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