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MORE ABOUT THIS BOOK
Main description:
A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.
Contents:
Part I. Overview
An Overview of Chemosensitivity Testing
Rosalyn D. Blumenthal
Part II. In Vitro Measures of Chemosensitivity
Clonogenic Cell Survival Assay
Anupama Munshi, Marvette Hobbs, and Raymond E. Meyn
High-Sensitivity Cytotoxicity Assays for Nonadherent Cells
M. Jules Mattes
Sulforhodamine B Assay and Chemosensitivity
Wieland Voight
Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity
Gertjan J. L. Kaspers
Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity
Hisayuki Kobayashi
The MTT Assay to Evaluate Chemosensitivity
Jack D. Burton
Histoculture Drug Response Assay to Monitor Chemoresponse
Shinji Ohie, Yasuhiro Udagawa, Daisuke Aoki, and Shiro Nozawa
In Vitro Testing of Chemosensitivity in Physiological Hypoxia
Rita Grigoryan, Nino Keshelava, Clarke Anderson, and C. Patrick Reynolds
Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements
Christian M. Kurbacher and Ian A. Cree
High-Throughput Technology: Green Fluorescent Protein to Monitor Cell Death
Marylene Fortin, Ann-Muriel Steff, and Patrice Hugo
DIMSCAN: A Microcomputer Fluorescence-Based Cytotoxicity Assay for Preclinical Testing of Combination Chemotherapy
Nino Keshelava, Tomasth Frgala, JirD i Krejsa, Ondrej Kalous, and C. Patrick Reynolds
The ChemoFx (R) Assay: An Ex Vivo Cell Culture Assay for Predicting Anticancer Drug Responses
Robert L. Ochs, Dennis Burholt, and Paul Kornblith
Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models
C. Patrick Reynolds and Barry J. Maurer
Image Analysis Using the Fluochromasia Assay to Quantify Tumor Drug Sensitivity
John F. Gibbs, Youcef M. Rustum, and Harry K. Slocum
ImmunohistochemicalDetection of Ornithine Decarboxylase as a Measure of Chemosensitivity
Uriel Bachrach
Immunohistochemistry of p53, Bcl-2 and Ki-67 as Predictors of Chemosensitivity
Mitsuyoshi Itaya, Jiro Yoshimoto, Kuniaki Kojima, and Seiji Kawasaki
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 245
Weight: 385g
Availability: Available
Subcategories: Oncology
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