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Main description:
In HaemophilusiInfluenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of H. influenzae. Described with step-by-instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. These methods illuminate how the bacterium causes disease, as well as how best to develop novel vaccines and antibiotics against the organism.
Contents:
The Pathogenesis of Disease Due to Nontypeable Haemophilus influenzae
Gail G. Hardy, Simone M. Tudor, and Joseph W. St. Geme, III.
The Pathogenesis of Disease Due to Type b Haemophilus influenzae
Ruth Aubrey and Christoph Tang
General Methods for Culturing Haemophilus influenzae
Grant Poje and Rosemary J. Redfield
Transformation of Haemophilus influenzae
Grant Poje and Rosemary J. Redfield
Diagnosis of Infection
Alex van Belkum and Loek van Alphen
Characterization of Plasmids
Ioanna D. Dimopoulou, Mohd-Zain Zaini, and Derrick W. Crook
Protein Characterization by Two-Dimensional Gel Electrophoresis
Phillip Cash and J. Simon Kroll
Monitoring Gene Expression Using DNA Arrays
Tahir R. Ali, Ming-Shi Li, and Paul R. Langford
Gene Expression Technology
Xavier de Bolle and Christopher D. Bayliss
The Genome Sequence of Haemophilus influenzae
Derek W. Hood
Structural Profiling of Short-Chain Lipopolysaccharides from Haemophilus influenzae
Elke K. H. Schweda and James C. Richards
Mutagenesis of H. influenzae
Mark A. Herbert
Transposon Tn10
Stefan Schloer, Gabriele Kemmer, and Joachim Reidl
In Vivo Expression of Bacterial Genes During Human Infections
Martin Handfield, Trevor Seifert, and Jeffrey D. Hillman
ELISA
Joyce S. Plested, Philip A. Coull, and Margaret Anne J. Gidney
Opsonophagocytosis Assay Using Flow-Cytometry
Joyce S. Plested and Philip A. Coull
In Vitro Models of Infection I-Human Respiratory Tissue Organ Culture
Andrew M. Middleton, Paula Keig, and Robert Wilson
n Vitro Models of Infection II-Human Umbilical Vein Endothelial Cells (HUVECs) System
Mumtaz Virji and Darryl J. Hill
Animal Models
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 348
Weight: 534g
Availability: Available
Subcategories: Infectious Diseases
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