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MORE ABOUT THIS BOOK
Main description:
A collection of new and essential molecular techniques for cardiovascular research. These readily reproducible methods range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using nonviral (polymers, liposomes, and antisense agents) and adenoviral vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. The book offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.
Contents:
Part I. Models of Hypertension
Congenic/Consomic Models of Hypertension
Delyth Graham, Martin W. McBride, Nicholas J. R. Brain, and Anna F. Dominiczak
Mouse Knockout Models of Hypertension
Michael Bader
Production of Transgenic Models in Hypertension
Elena Popova, Michael Bader, and Alexander Krivokharchenko
Measuring Blood Pressure in Small Laboratory Animals
Klaas Kramer and Rene Remie
Part II. Assessment of Free Radicals in Endothelial Dysfunction
Analysis of Superoxide Anion Production in Tissue
Michela Zanetti, Livius V. d'Uscio, Timothy E. Peterson, Zvonimir S. Katusic, and Timothy O'Brien
Measurement of Vascular Reactive Oxygen Species Production by Chemiluminescence
Tomasz J. Guzik and Keith M. Channon
A Guide to Wire Myography
Angela Spiers and Neal Padmanabhan
Part III. Nucleic Acid Techniques
Selection of Candidate Genes in Hypertension
Charles A. Mein, Mark J. Caulfield, and Patricia B. Munroe
Genome-Wide Scanning With SSLPs in the Rat
Carol Moreno, Kathleen Kennedy, Jaime Wendt Andrae, and Howard J. Jacob
Extraction of RNA From Cells and Tissue
Ian M. Bird
Single-Strand Conformational Polymorphism Analysis: Basic Principles and Routine Practice
Yanbin Dong and Haidong Zhu
Single-Nucleotide Polymorphism Genotyping for Disease Association Studies
Myriam Fornage and Peter A. Doris
Denaturing High-Performance Liquid Chromatography Using the WAVE DNA Fragment Analysis System
Elizabeth Donohoe
TaqMan Real-Time PCR Quantification: Conventional and Modified Methods
Michihiro Yoshimura, Shota Nakamura, and Hisao Ogawa
Generation of High-Sensitivity Antisense cDNA Probes by Asymmetric PCR
Ian M. Bird
New Advances in Microarrays: Finding the Genes Causally Involved in Disease
ClaudioNapoli, Filomena de Nigris, and Vincenzo Sica
Pharmacogenetics and Pharmacogenomics
Klaus Lindpaintner
Part IV. Proteins and Proteomics
Determination of the Endogenous Nitric Oxide Synthase Inhibitor Asymmetric Dimethylarginine in Biological Samples by HPLC
Tom Teerlink
Proteomic Approaches in the Analysis of Hypertension
Soren Naaby-Hansen, Gayathri D. Warnasuriya, Claire Hastie, Piers Gallney, and Rainer Cramer
Part V. Gene Transfer
Gene Transfer in Endothelial Dysfunction and Hypertension
Sabyasachi Sen, Padraig M. Strappe, and Timothy O'Brien
Nonviral Gene Delivery Methods in Cardiovascular Diseases
Marika Ruponen, Zanna Hyvoenen, Arto Urtti, and Seppo Yla-Herttuala
Construction and Characterization of Helper-Dependent Adenoviral Vectors for Sustained In Vivo Gene Therapy
Kazuhiro Oka and Lawrence Chan
Adenovirus-Mediated Gene Transfer In Vivo: An Approach to Reduce Oxidative Stress
Yi Chu
Gene Therapy for Hypertension: Antisense Inhibition of the Renin-Angiotensin System
M. Ian Phillips and Birgitta Kimura
Modulation of Gene Expression by RNAi
Cezary Wojcik, Rosalind Fabunmi, and George N. DeMartino
In Vivo Biopanning: A Methodological Approach to Identifying Novel Targeting Ligands for Delivery of Biological Agents to the Vasculature
Lorraine M. Work, Campbell G. Nicol, Laura Denby, and Andrew H. Baker
Part VI. Stem Cells
Cardiomyocytes Derived From Embryonic Stem Cells
Kenneth R. Boheler, David G. Crider, Yelena Tarasova, and Victor A. Maltsev
Part VII. Bioinformatics
In Silico and Wet-Bench Identification of Nuclear Matrix Attachment Regions
Stephen A. Krawetz, Sorin Draghici, Robert Goodrich, Zhandong Liu, and G. Charles Ostermeier
Biomedical Informatics Methods in Pharmacogenomics
Qing Yan
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 518
Weight: 757g
Availability: Available
Subcategories: Cardiovascular Medicine
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