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Main description:
A cutting-edge collection of readily reproducible in vitro and in vivo methods to elucidate the mechanisms associated with cannabinoid function in health and disease. The techniques can be used in studies across the board from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure, the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their association with polysubstance abuse. There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid effects using brain slice imaging and elctrophysiological approaches, and designing and synthesizing cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Contents:
Molecular Neurobiological Methods in Marijuana-Cannabinoid Research
George R. Uhl, Hiroki Ishiguro, Emmanuel S. Onaivi, Zhicheng Lin, B. Emmanuel Akinshola, Bruce Hope, Claire M. Leonard, and Qing-Rong Liu
Experimental Methods to Study the Role of the Peripheral Cannabinoid Receptor in Immune Function
Nancy E. Buckley, Diep Burbridge, Manop Buranapramest, Tanya Ferguson, and Renee Y. Paau
Localization of Cannabinoid Receptors Using Immunoperoxidase Methods
Guy A. Cabral
Localization of Cannabinoid CB1 Receptor mRNA Using Ribonucleotide Probes: Methods for Double- and Single-Label In Situ Hybridization
Andrea G. Hohmann
Morphometric Study on Cytoskeletal Components of Neuronal and Astroglial Cells After a Chronic CB1 Agonist Treatment
Patricia Tagliaferro, Alberto J. Ramos, Emmanuel S. Onaivi, Sergio G. Evrard, Maite Duhalde Vega, and Alicia Brusco
Visualizing Cannabinoid Effects Using Brain Slice Imaging and Electrophysiological Approaches
Alexander F. Hoffman and Carl R. Lupica
Methods for the Synthesis of Cannabinergic Ligands
Ganesh A. Thakur, Spyros P. Nikas, Richard I. Duclos, Jr., and Alexandros Makriyannis
Cannabinoid Receptor Binding to Membrane Homogenates and Cannabinoid-Stimulated [35S]GTPgS Binding to Membrane Homogenates or Intact Cultured Cells
Chris Breivogel
Methods to Assay Anandamide Hydrolysis and Transport in Synaptosomes
Filomena Fezza, Natalia Battista, Monica Bari, and Mauro Maccarrone
Methods Evaluating Cannabinoid and Endocannabinoid Effects on Gastrointestinal Functions
Nissar A. Darmani
The Bioassay of Cannabinoids Using the Mouse Isolated Vas Deferens
Ad`ele Thomas and Roger G. Pertwee
Assessment of Cannabis Craving Using the Marijuana Craving Questionnaire
Stephen J. Heishman and Edward G. Singleton
Methods for Studying Acute and Chronic Effects ofMarijuana on Human Associative Processes and Memory
Robert I. Block
Methods for Clinical Research Involving Cannabis Administration
David A. Gorelick and Stephen J. Heishman
Neurological Assessments of Marijuana Users
Jean Lud Cadet, Karen Bolla, and Ronald I. Herning
Behavioral Methods in Cannabinoid Research
Ester Fride, Alex Perchuk, F. Scott Hall, George R. Uhl, and Emmanuel S. Onaivi
Methods to Study the Behavioral Effects and Expression of CB2 Cannabinoid Receptors and Its Gene Transcripts in Chronic Mild Stress Model of Depression
Emmanuel S. Onaivi, Hiroki Ishiguro, Patel Sejal, Paul A. Meozzi, Lester Myers, Patricia Tagliaferro, Bruce Hope, Claire M. Leonard, George R. Uhl, Alicia Brusco, and Eileen Gardner
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 324
Weight: 478g
Availability: Available
Subcategories: Pharmacology
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