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Main description:
Tumor necrosis factor (TNF)-? is a pleiotropic cytokine involved in a va- ety of physiological and pathological processes. After initial discovery of its ability to induce cell death and animal cachexia, it was soon realized that this cytokine played pivotal roles in the regulation of homeostasis and inflam- tory-immune responses. This led to an explosion of interest in basic and tra- lational research activities on the role of TNF in many diseases, such as cancer, septic shock, rheumatoid arthritis, and infectious diseases of the central n- vous system. Because of its potential therapeutic value, many academic and industrial research groups have worked to discover compounds that can block its activity. These studies have led to the approval of anti-TNF antibodies and soluble TNF receptors for the therapy of rheumatoid arthritis and Crohn's d- ease. TNF also can be an attractive anticancer agent capable of damaging tum- associated vessels and of inducing tumor necrosis in patients.
The unique properties of TNF have led to its registration as a drug for locoregional tre- ment of sarcomas of the extremities, and stimulated many preclinical studies aimed at improving its therapeutic index for systemic use. Tumor Necrosis Factor: Methods and Protocols provides an overview of basic and translational research along with a series of practical procedures on TNF production, characterization, mutagenesis, detection in biological spe- mens, as well as several in vitro assays and animal models for studying the role of TNF in various diseases.
Contents:
Tumor Necrosis Factor as a Pharmacological Target
Pietro Ghezzi and Anthony Cerami
Production and Characterization of Recombinant Human and Murine TNF
Flavio Curnis and Angelo Corti
Purification of TNF Binding Proteins
Hartmut Engelmann, Dani Aderka, and David Wallach
Production and Characterization of Receptor-Specific TNF Muteins
Paul Ameloot and Peter Brouckaert
TNFa Promoter Polymorphysms
Silvia Fargion, Luca Valenti, Paola Dongiovanni, and Anna Ludovica Fracanzani
Detection of TNF and TNF Receptor mRNA in Cells and Tissues
Roberto Furlan, Giuliana Salani, Alessandra Bergami, and Gianvito Martino
Immunohistochemical Localization of TNFa and Its Receptors in the Rodent Central Nervous System
Paolo Bigini and Tiziana Mennini
TNF Signaling: Key Protocols
Marta Muzio and Simona Saccani
More Than One Way to Die: Methods to Determine TNF-Induced Apoptosis and Necrosis
Tom Vanden Berghe, Geertrui Denecker, Greet Brouckaert, Dmitri Vadimovisch Krysko, Katharina D'Herde, and Peter Vandenabeele
Assessment of TNFa-Induced Endothelial Damage Through the Loss of Its Barrier Function
Elisabetta Ferrero
Genetic Engineering in the Mouse: Tuning TNF/TNFR Expression
Eleni Douni, Maria Alexiou, and George Kollias
TNFa in Experimental Diseases of the CNS
Roberto Furlan, Pia Villa, Giorgio Senaldi, and Gianvito Martino
In Vivo Vascular Leakage Assay
Maria Elena Ferrero
Animal Models of Endotoxic Shock
Pia Villa and Pietro Ghezzi
Collagen-Induced Arthritis as a Model for Rheumatoid Arthritis
Richard O. Williams
Myocardial Infarction: Animal Models
Stefano Chimenti, Eleonora Carlo, Serge Masson, Antonio Bai, and Roberto Latini
Tumor Vascular Therapy With TNF: Critical Review on Animal Models
Timo L. M. ten Hagen and Alexander M.M. Eggermont
Strategies for Improving the Anti-Neoplastic Activity of TNF by Tumor Targeting
Angelo Corti
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 292
Weight: 433g
Availability: Available
Subcategories: Oncology
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