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Main description:
Elizabeth Rakcozy and a team of leading clinical and experimental scientists describe in step-by-step detail the key techniques essential to effective molecular biological research in ophthalmology and optometry. These readily reproducible methods are adapted to the special requirements of vision research, with coverage that ranges from the most basic to the most sophisticated technologies. Included are methods for the down-regulation of gene expression, new gene therapy techniques, and for the development of transgenic and knockout animal models for testing novel therapies. Eminently accessible and clinically relevant, Vision Research Protocols provides experimental and biomedical investigators in ophthalmology and optometry with a rich panoply of most powerful tools with which to ask--and answer--all the important questions emerging from the dramatically advancing work in vision research today.
Contents:
Basic Molecular Biology Techniques, Chooi-May Lai. Transient Transfection of Human Retinoblastoma Cells: Application to the Analysis of the Regulatory Regions of Photoreceptor-Specific Genes, Debora B. Farber, Leonid E. Lerner, and Andrea S. Viczian. In Situ Hybridization, Xian-Jie Yang. Immunocytochemical Studies of the Retina, Ann H. Milam. Antisense DNA Technology, Piroska Elizabeth Rakoczy. Inhibition of Gene Expression by Ribozymes, William W. Hauswirth, Lynn C. Shaw, Patrick O. Whalen, Jason J. Fritz, D. Alan White, and Alfred S. Lewin. Intraocular Delivery of Recombinant Virus, Fong-Qi Liang, Vibha Anand, Albert M. Maguire, and Jean Bennett. Preparation of Recombinant Adenoviruses, Karl G. Csaky. Generation of Recombinant Adeno-Associated Virus, Fabienne Rolling. Preparation of Recombinant Retroviruses, Xian-Jie Yang. Generation of Transgenic Mice for Studies of Ocular Development and Disease, Abha R. Gupta, Nadine S. Dejneka, Albert M. Maguire, and Jean Bennett. Generation of Knockout Animal Models, T. Michael Redmond. Mutation Analysis of Ocular Genes, Michael Danciger. Ophthalmologists' Perspective, Susan M. Downes, N. H. Victor Chong, and Alan C. Bird. Index.
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: May, 2011
Pages: 330
Weight: 487g
Availability: Available
Subcategories: Ophthalmology and Optometry
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