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Main description:
Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it. In their discussion of research methodologies utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Contents:
Part I. The Clinical Scope of Fibrosis
Pulmonary Fibrosis
David A. Zisman, Michael P. Keane, John A. Belperio, Robert M. Strieter, and Joseph P. Lynch, III
Renal Fibrosis
H. William Schnaper
Transforming Growth Factor-b: A Key Mediator of Fibrosis
Alain Mauviel
Part II. Regulation of Extracellular Matrix Metabolism: Cultured Cells
Isolation and Culture of Skin Fibroblasts
Laure Rittie and Gary J. Fisher
Isolation and Culture of Hepatic Stellate Cells
Ralf Weiskirchen and Axel M. Gressner
Isolation and Phenotypic Characterization of Lung Fibroblasts
Carolyn J. Baglole, Sireesha Y. Reddy, Stephen J. Pollock, Steven E. Feldon, Patricia J. Sime, Terry J. Smith, and Richard P. Phipps
Methods for Measuring Type I Collagen Synthesis In Vitro
David C. Rishikof, Ping-Ping Kuang, Mangalalaxmy Subramanian, and Ronald H. Goldstein
Methods for Assessing the Molecular Mechanisms Controlling Gene Regulation
Richard A. Rippe and Branko Stefanovic
Methods for Measuring TGF-b Using Antibodies, Cells, and Mice
Vladimir Jurukovski, Branka Dabovic, Vesna Todorovic, Yan Chen, and Daniel B. Rifkin
Part III. Studying Fibrosis Using Animal Models
Morphological Methods for Assessment of Fibrosis
Rakesh K. Kumar
Methods for Measuring Hydroxyproline and Estimating In Vivo Rates of Collagen Synthesis and Degradation
Robin J. McAnulty
Approaches to Evaluation of Fibrogenic Pathways in Surgical Lung Biopsy Specimens
Cory M. Hogaboam, Kristin J. Carpenter, Holly Evanoff, and Steven L. Kunkel
Animal Models for Adult Dermal Wound Healing
Mary Birch, Annette Tomlinson, and Mark W. J. Ferguson
Modeling Liver Fibrosis in Rodents
Christothea Constandinou, Neil Henderson, and John P. Iredale
Animal Models of Pulmonary Fibrosis
MehrnazGharaee-Kermani, Matthew Ullenbruch, and Sem H. Phan
Animal Models of Renal Fibrosis
Michael Zeisberg, Mary A. Soubasakos, and Raghu Kalluri
Animal Models of Cardiac Fibrosis
Yao Sun and Karl T. Weber
Part IV. Genetic Approaches to Studying Fibrosis
Finding Fibrosis Genes: The Lung
Lauranell H. Burch and David A. Schwartz
Genetic Studies to Identify Hepatic Fibrosis Genes and SNPs in Human Populations
Christopher Paul Day
Analysis of Microarray Experiments for Pulmonary Fibrosis
Nilesh B. Dave and Naftali Kaminski
DNA Microarrays and Data Mining to Study Hepatic Fibrosis
Bernd Schnabl, Youkyung Hwang Choi, Curt H. Hagedorn, and Ramon Bataller
Analysis of Proteins Dominantly Expressed in Hepatic Stellate Cells of Activated Phenotype: The Molecular Approach for Liver Fibrosis
Norifumi Kawada
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 410
Weight: 600g
Availability: Available
Subcategories: Respiratory Medicine
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