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MORE ABOUT THIS BOOK
Main description:
Diabetes mellitus is the collective name for a group of diseases associated with hyperglycemia (high levels of blood glucose) caused by defects in insulin p- duction, insulin action, or both. About 6. 2% of the US population (17 million people) have diabetes mellitus. It is the leading cause of kidney failure, bli- ness, and amputations. It is also a major risk factor for heart diseases, stroke, and birth defects. Diabetes Mellitus: Methods and Protocols provides a state-of-the-art account of the experimental methodology for studying the molecular defects leading to diabetes mellitus, both at the molecular and biochemical levels. The chapters cover a wide range of topics written by experts in their respective fields and are organized in two sections: Insulin Production and Insulin Action. The detailed experimental protocols presented, including the notes of interest, provide a very useful tool for basic researchers and clinicians for investigating and treating this disease. Each chapter starts with an introduction to a specific technique and explains its application in the field of diabetes research. Following the list of materials, a detailed description of the technique is presented in the methods section in a way that enables the successful execution of the protocol. The "Notes" section at the end discusses the pitfalls of the technique and alternative approaches. I am grateful to the numerous scientists who have contributed to this volume by writing both highly detailed and understandable chapters.
Contents:
Part I. Insulin Production
Isolation of Islets of Langerhans from Rodent Pancreas
Colleen B. Kelly, Libby A. Blair, John A. Corbett, and Anna L. Scarim
Purification of Rat Pancreatic b-Cells by Fluorescence-Activated Cell Sorting
Geert Stange, Mark Van De Casteele, and Harry Heimberg
Assessment of Insulin Secretion in the Mouse
Marcela Brissova, Wendell E. Nicholson, Masakazu Shiota, and Alvin C. Powers
Detection of Insulin Production by Immunohistochemistry
Susan C. Campbell and Wendy M. Macfarlane
Quantification of the Level of Insulin Gene Expression
Susan C. Campbell and Wendy M. Macfarlane
Chromatin Immunoprecipitation Using Isolated Islets of Langerhans
Marcelina Parrizas, Sylvia F. Boj, Reini F. Luco, Miguel Angel Maestro, and Jorge Ferrer
Adenoviral Gene Transfer into b-Cell Lines
Amber L. Mosley and Sabire OEzcan
Utilization of NOD Mice in the Study of Type 1 Diabetes
Dennis G. Karounos and Susan E. Goes
Part II. Insulin Action
Introduction of DNA into 3T3-L1 Adipocytes by Electroporation
Shuichi Okada, Masatomo Mori, and Jeffrey E. Pessin
Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes
Jaganathan Lakshmanan, Jeffrey S. Elmendorf, and Sabire OEzcan
Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes
Jeffrey S. Elmendorf
Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay
John B. Knight and Ann Louise Olson
Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates
Eileen L. Whiteman and Morris J. Birnbaum
Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity
Li-Ping Wang and Scott A. Summers
Assaying AKT/ Protein Kinase B Activity
Kyle L. Hoehn and Scott A.Summers
Measurements of Cellular Phosphoinositide Levels in 3T3-L1 Adipocytes
Daryll B. DeWald
Measurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 Adipocytes
Matthew J. Brady
Measurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal Muscle
Joseph T. Brozinick, Jr.
Single Embryo Measurement of Basal- and Insulin-Stimulated Glucose Uptake
Maggie M-Y. Chi and Kelle H. Moley
Immunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of Glucose Transporters
Mary O. Carayannopoulos and Kelle H. Moley
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 208
Weight: 309g
Availability: Available
Subcategories: Endocrinology
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