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Main description:
There is now a range of cytotoxic drugs that have considerable clinical usefulness in producing responses in tumors and even, in a small proportion of cases, cure. However, the acquisition of drug resistance is a major clinical problem and is perhaps the main limiting factor in successful treatment of cancer. Thus, a tumor initially sensitive to chemotherapy will, in the majority of cases, eventually recur as a resistant tumor, which will then progress. Much of our understanding of drug resistance mechanisms comes from the study of tumor cell lines grown in tissue culture. We now understand many of the - lecular mechanisms that can lead to a cell acquiring resistance to antic- cer drugs; however, we still do not know which mechanism(s) are those most relevant to the problem of clinical drug resistance. Indeed, given that many of the cytotoxic anticancer drugs were discovered by random screening, it is - clear what features give a clinically useful anticancer drug a sufficient the- peutic index to be of value. The aim of Cytotoxic Drug Resistance Mechanisms is to provide pro- cols that are appropriate for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumor samples. Tumor cell lines have been enormously useful as experimental models of drug resistance mechanisms, however they have limitations and we need to address the relevance of such mechanisms in patients' tumors. Examining drug resistance in tumors is much more problematic than in cell lines.
Contents:
Drug Resistance: The Clinical Perspective
D. Alan Anthoney and Stanley B. Kaye
Cell Sensitivity Assays: Clonogenic Assay
Jane A. Plumb
Cell Sensitivity Assays: The MTT Assay
Jane A. Plumb
Cell Sensitivity Assays: Detection of Apoptotic Cells In Vitro Using the TUNEL Assay
Neil A. Jones and Caroline Dive
Analysis of Apoptosis in Tissue Sections
Vicki Save, Philip J. Coates, and Peter A. Hall
Measurement of P-glycoprotein Function
Henk J. Broxterman
Measuring MDR-1 by Quantitative RT-PCR
Susan E. Bates, Zhirong Zhan, Joanna Regis, and Erick Gamelin
Microtiter Plate Technique for the Measurement of Glutathione in Fresh and Cryopreserved Lymphoblasts Using the Enzyme Recycling Method
Pamela R. Kearns and Andrew G. Hall
Measurement of Reduced Glutathione Using High Pressure Liquid Chromatography
Linda A. Hogarth, Celia M. A. Rabello, and Andrew G. Hall
Topoisomerase I and II Activity Assays
Philippe Pourquier, Glenda Kohlhagen, Li-Ming Ueng, and Yves Pommier
5-Fluorouracil Metabolizing Enzymes
Howard L. McLeod, Lesley H. Milne, and Stephen J. Johnston
Measuring DNA Adducts by Immunoassay (ELISA)
Michael J. Tilby
Measuring Drug-DNA Adducts in Individual Cells
Adrian J. Frank
Measurement of Drug-Induced DNA Interstrand Crosslinking Using the Single Cell Electrophoresis (Comet) Assay
Victoria J. Spanswick, Janet M. Hartley, Timothy H. Ward, and John A. Hartley
PCR Analysis of Microsatellite Instability
Gillian L. Hirst
O 6-Alkylguanine-DNA Alkyltransferase Assay
Amanda J. Watson and Geoffrey P. Margison
Analysis of the p53 Status of Tumors: An Overview of Methods
Jonas Bergh
Bcl-2 Family Immunohistochemistry
Lloyd R. Kelland and Philip J. Beale
Genetic Analysis of Drug Resistance by Fluorescence In Situ Hybridization
W. Nicol Keith
Genetic Analysis of Drug Resistance by ReverseIn Situ Hybridization
W. Nicol Keith
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 248
Weight: 376g
Availability: Available
Subcategories: Oncology
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