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Main description:
John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus (CMV) biology and its pathogenetic mechanisms. The methods-all described in step-by-step detail with ready reproducibility in mind-range from basic virus culture to complex molecular analysis of CMV structure and function. Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host. Comprehensive and state-of-the-art, Cytomegalovirus Protocols provides investigators with a collection of the key methods that are illuminating not only the basic biology of this complex and intriguing human herpesvirus, but also its significant role in human infectious diseases and their emergent therapies.
Contents:
Immunological Methods For The Dectection of Human Cytomegalovirus, Andrew D. Yurochko and Eng-Shang Huang. Identification of Immunoreactive Viral Proteins, Walter Hinderer, Bodo Plachter, and Rolf Vornhagen. Analysis of Human Cytomegalovirus Using The Polymerase Chain Reaction, Marc Mendelson. Analysis of Cytomegalovirus Ligands, Receptors, and the Entry Pathway, Teresa Compton. Analysis of Cytomegalovirus Gene Expression by Transfection, Anamaris M. Colberg-Poley, Vera E. Soltero, and Richard-Fabian Schumacher. Analysis of Protein-Protein Interactions During Cytomegalovirus Infection, Richard C. Caswell. Analysis of Protein-DNA Interactions During Cytomegalovirus Infection, Stefan FloeB and Thomas Stamminger. Analysis of Cytotoxic T-Cell Responses to Cytomegalovirus Infection, Mark R. Wills and J G Patrick Sissons. Methods in Anti-HCMV Research, Johan Neyts, Graciela Andrei, Robert Snoeck, Astrid Meerbach, and E.rik De Clercq. Construction of Recombinant Human Cytomegalovirus, Richard F. Greaves.
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2011
Pages: 192
Weight: 288g
Availability: Available
Subcategories: General Issues, Infectious Diseases
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