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Main description:
Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Contents:
Noncontact Laser Microdissection and Pressure Catapulting: Sample Preparation for Genomic, Transcriptomic, and Proteomic Analysis
Yilmaz Niyaz, Monika Stich, Bernd Sagmuller, Renate Burgemeister, Gabriele Friedemann, Ulrich Sauer, Rainer Gangnus, and Karin Schutze
Applications of the Universal DNA Microarray in Molecular Medicine
Reyna Favis, Norman P. Gerry, Yu-Wei Cheng, and Francis Barany
Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization
Zhi-wei Zhang, Yi-ming Zhou, Yan Zhang, Yong Guo, Sheng-ce Tao, Ze Li, Qiong Zhang, and Jing Cheng
Genotyping Single-Nucleotide Polymorphisms by Minisequencing Using Tag Arrays
Lovisa Lovmar and Ann-Christine Syvanen
Single-Nucleotide Polymorphism and Mutation Identification by the Nanogen Microelectronic Chip Technology
Maurizio Ferrari, Laura Cremonesi, Pierangelo Bonini, Barbara Foglieni, and Stefania Stenirri
Molecular Diagnostic Testing for Inherited Thrombophilia Using Invader (R)
Margaret A. Keller
Controlled Agitation During Hybridization: Surface Acoustic Waves Are Shaking Up Microarray Technology
Achim Wixforth
Rapid Screening for 31 Mutations and Polymorphisms in the Cystic Fibrosis Transmembrane Regulator Gene by Luminex (R) xMAP (TM) Suspension Array
Sherry A. Dunbar and James W. Jacobson
Protein Microarray-Based Screening of Antibody Specificity
Rhonda Bangham, Gregory A. Michaud, Barry Schweitzer, and Paul F. Predki
Multiplexed Protein Analysis Using Antibody Microarrays and Label-Based Detection
Brian B. Haab
Allergen Microarrays
Tito Bacarese-Hamilton, Julian Gray, Andrea Ardizzoni, and Andrea Crisanti
Enhanced Protein Profiling Arrays for Quantitative Measurement of Protein Expression in Multiple Samples
Ruochun Huang, Qian Shi,Weimin Yang, and Ruo-Pan Huang
Multiplexed Cytokine Sandwich Immunoassays: Clinical Applications
Uma Prabhakar, Edward Eirikis, Bruce E. Miller, and Hugh M. Davis
Validation and Quality Control of Protein Microarray-Based Analytical Methods
Larry J. Kricka and Stephen R. Master
Tissue Microarrays
Ronald Simon, Martina Mirlacher, and Guido Sauter
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 290
Weight: 430g
Availability: Available
Subcategories: Pathology
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