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MORE ABOUT THIS BOOK
Main description:
Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs – of viral or cellular origin – affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.
Feature:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs – of viral or cellular origin – affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.
Contents:
Part I: General Introduction
1. Defense and counter-defense in the RNAi-based antiviral immune system in insects
Joël T. van Mierlo, Koen W.R. van Cleef, and Ronald P. van Rij
2. RNAi and cellular miRNAs in infections by mammalian viruses
Joost Haasnoot and Ben Berkhout
3. Viral miRNAs
Karlie Plaisance-Bonstaff, and Rolf Renne
4. Progress in RNAi-based antiviral therapeutics
Jiehua Zhou and John J. Rossi
5. Chemical modification of small interfering RNA
Jesper B. Bramsen and Jørgen Kjems
Part II: Viral small RNAs
6. Viral small RNA cloning and sequencing
Valérie Gausson and Maria-Carla Saleh
7. Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets
Christophe Antoniewski
8. Computational prediction of viral miRNAs
Adam Grundhoff
9. Detection of Viral microRNAs by Northern Blot Analysis
Lydia V. McClure, Yao-Tang Lin, and Christopher S. Sullivan
10. Detection of viral microRNA with S1 Nuclease Protection assay
Matthias John and Sébastien Pfeffer
11. Characterization of RISC associated adenoviral small RNAs
Ning Xu and Göran Akusjärvi
Part III: RNAi-based antiviral defense
12. Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture
Koen W.R. van Cleef, Joël T van Mierlo, Marius van den Beek, and Ronald P. van Rij
13. Dicer assay in Drosophila S2 cell extract
Baojun Yang and Hongwei Li
14. Slicer activity in Drosophila melanogaster S2 extract
Arabinda Nayak and Raul Andino
15. Gel Mobility Shift Assays for RNA-binding viral RNAi suppressors
Tibor Csorba and József Burgyán
16. dsRNA uptake in adult Drosophila
Benjamin Obadia and Maria-Carla Saleh
Part IV: Antiviral RNAi therapy
17. Design of small interfering RNAs for antiviral applications
Diana Rothe, Erik J. Wade, and Jens Kurreck
18. RNAi inducing lentiviral vectors for anti-HIV-1 gene therapy
Ying Poi Liu, Jan-Tinus Westerink, Olivier ter Brake, and Ben Berkhout
19. Production of multicopy shRNA lentiviral vectors for antiviral therapy
Scot D. Henry, Quiwei Pan, and Luc J.W. van der Laan
20. Intranasal delivery of antiviral siRNA
Sailen Barik
21. Antibody mediated delivery of siRNAs for anti-HIV therapy
Sang-Soo Kim, Sandesh Subramanya, Dan Peer, Motomu Shimaoka, and Premlata Shankar
22. Aptamer-targeted RNAi for HIV-1 therapy
Jiehua Zhou and John J. Rossi
Part V: RNAi screens to study virus-host interactions
23. RNAi screening for host factors involved in viral infection using Drosophila cells
Sara Cherry
24. Genome-wide RNAi Screen for Viral Replication in Mammalian Cell Culture
Bhupesh K. Prusty, Alexander Karlas, Thomas F. Meyer, and Thomas Rudel
25. RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infections
Carolyn B. Coyne and Sara Cherry
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: March, 2011
Pages: 355
Weight: 986g
Availability: Not available (reason unspecified)
Subcategories: Genetics, Microbiology
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CUSTOMER REVIEWS
From the reviews:
“This well-illustrated book details the necessary protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as the development and optimization of RNAi-based antiviral drugs. The book is intended for both novice and experienced researchers … . It also will be of immense help to graduate students, physicians who treat patients with infectious diseases, and other researchers interested in the field of antiviral RNAi. … a well-illustrated and authoritative resource on important basic concepts in the field of antiviral RNAi.” (Omer Iqbal, Doody’s Review Service, July, 2011)