(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.
Contents:
Section One: TECHNOLOGIES
Chapter One - Energy Systems for ATP Regeneration in Cell-free Protein Synthesis Reactions
Kara A. Calhoun, James R. Swartz
Chapter 2 - Continuous-exchange Protein-synthesizing Systems
Vladimir A. Shirokov, Aigar Kommer, Vyacheslav A. Kolb, Alexander S. Spirin
Chapter 3 - Cell-free production of integral membrane proteins on a preparative scale'
Christian Klammt, Daniel Schwarz, Vilker Doetsch, Frank Bernhard
Chapter 4- SIMPLEX: Single-molecule PCR-linked in vitro expression: a novel method for high-throughput construction and screening of protein libraries
Suang Rungpragayphan, Tsuneo Yamane, Hideo Nakano
Chapter 5 - Methods for High-throughput Materialization of Genetic Information Based on Wheat Germ Cell-free Expression System
Tatsuya Sawasaki, Ryo Morishita, Mudeppa D. Gouda, Yaeta Endo
Chapter 6 - Exogenous Protein Expression in Xenopus Oocytes: Basic Procedures
Elena Bossi, Maria Serena Fabbrini, Aldo Ceriotti
Section Two: APPLICATIONS
Chapter 7 -In vitro translation to study HIV protease activity
Zene Matsuda, Mutsunori Iga, Kosuke Miyauchi, Jun Komano, Kazuhiro Morishita, Akihiko Okayama, Hirohito Tsubouchi
Chapter 8 - The protein truncation test in mutation detection and molecular diagnosis
Oliver Hauss, Oliver Muller
Chapter 9 - Creation of Novel Enantioselective lipases by SIMPLEX
Yuichi Koga, Tsuneo Yamane, Hideo Nakano
Chapter 10 - In vitro transcription and translation coupled to 2D-electrophoresis for bacterial proteome analysis
Nathalie Norais, Ignazio Garaguso, Germano Ferrari, Guido Grandi
Chapter 11 - In vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation and Immunoprecipitation Analysis
Orit Gat, Haim Grosfeld,Avigdor Shafferman
Chapter 12 - Functional expression of type 1 rat GABA transporter in microinjected Xenopus laevis oocytes
Stefano Giovannardi, Simona Magagnin, Andrea Soragna, Laura Faravelli
Chapter 13 - Production of Protein for NMR Study Using the Wheat Germ Cell-Free System
Toshiyuki Kohno, Yaeta Endo
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2010
Pages: 290
Weight: 425g
Availability: Available
Subcategories: Biochemistry, Genetics
Publisher recommends
From the same series
