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Main description:
Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics. In second edition of Laser Capture Microdissection: Methods and Protocols, experts in the field provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies. The individual chapters encompass detailed descriptions of the individual laser based micro-dissection systems. The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis including application to microarrays and proteomics. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Protocols, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology.
Contents:
1. Laser Capture Microdissection: Methods and Applications
Kristen DeCarlo, Andrew Emley, Ophelia E. Dadzie, and Meera Mahalingam
2. Laser Microdissection for Gene Expression Profiling
Lori A. Field, Brenda Deyarmin, Craig D. Shriver, Darrell L. Ellsworth, and Rachel E. Ellsworth
3. Gene Expression Using the PALM System
Jian-Xin Lu and Cheuk-Chun Szeto
4. Immuno-Guided Microdissection Techniques
Michael A. Tangrea, Jeffrey C. Hanson, Robert F. Bonner, Thomas J. Pohida, Jaime Rodriguez-Canales, and Michael R. Emmert-Buck
5. Optimized RNA Extraction from Non-Deparaffinized, Laser-Microdissected Material
Danny Jonigk, Friedrich Modde, Clemens Bockmeyer, Jan Becker, and Ulrich Lehmann
6. Laser Capture Microdissection for Analysis of Gene Expression in Formalin-Fixed Paraffin-Embedded Tissue
Ru Jiang, Rona S. Scott, and Lindsey M. Hutt-Fletcher
7. MicroRNA Profiling Using RNA from Microdissected Immunostained Tissue
Clemens L. Bockmeyer, Danny Jonigk, Hans Kreipe, and Ulrich Lehmann
8. Profiling Solid Tumor Heterogeneity by LCM and Biological MS of Fresh Frozen Tissue Sections
Donald J. Johann, Jr., Sumana Mukherjee, DaRue A. Prieto, Timothy D. Veenstra, and Josip Blonder
9. Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification of Microdissected Tissue
Cathy B. Moelans, Roel A. de Weger, and Paul J. van Diest
10. Detection and Quantification of MicroRNAs in Laser Microdissected Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues
Sarkawt M. Khoshnaw, Des G. Powe, Ian O. Ellis, and Andrew R. Green
11. Laser Capture Microdissection Applications in Breast Cancer Proteomics
Rene B.H. Braakman, Theo M. Luider, John W.M. Martens, John A. Foekens, and Arzu Umar
12. Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS
Isabelle Cadron, Toon Van Gorp, Philippe Moerman, Etienne Waelkens, and Ignace Vergote
13. LCM Assisted Biomarker Discovery from Archival Neoplastic Gastrointestinal Tissues
Patricia A. Meitner and Murray B. Resnick
14. Purification of Diseased Cells from Barrett's Esophagus and Related Lesions by Laser Capture Microdissection
Masood A. Shammas and Manjula Y. Rao
15. Laser Microdissection of Intestinal Epithelial Cells and Downstream Analysis
Benjamin Funke
16. Application of Laser Microdissection and Quantitative PCR to Assess the Response of Oesophageal Cancer to Neoadjuvant Chemo-Radiotherapy
Claus Hann von Weyhern and Bjoern L.D.M. Brucher
17. Oligonucleotide Microarray Expression Profiling of Contrasting Invasive Phenotypes in Colorectal Cancer
Christopher C. Thorn, Deborah Williams, and Thomas C. Freeman
18. Evaluation of Gastrointestinal mtDNA Depletion in Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE)
Carla Giordano and Giulia d'Amati
19. Laser Microdissection for Gene Expression Study of Hepatocellular Carcinomas Arising in Cirrhotic and Non-Cirrhotic Livers
Maria Tretiakova and John Hart
20. Laser Capture Microdissection of Pancreatic Ductal Adeno-Carcinoma Cells to Analyze EzH2 by Western Blot Analysis
Aamer M. Qazi, Sita Aggarwal, Donald W. Weaver, and Ramesh B. Batchu
21. Laser-Capture Microdissection of Renal Tubule Cells and Linear Amplification of RNA for Microarray Profiling and Real-Time PCR
Susie-Jane Noppert, Susanne Eder, and Michael Rudnicki
22. Subcellular Renal Proximal Tubular Mitochondrial Toxicity with Tenofovir Treatment
James J. Kohler and Seyed H. Hosseini
23. Application of Laser Capture Microdissection to Study Renal Carcinogenesis
Kerstin Stemmer and Daniel R. Dietrich
24. Laser Capture Microdissection and Transcriptional Profiling in Archival FFPE Tissue in Prostate Cancer
Ajay Joseph and Vincent J. Gnanapragasam
25. Quantitative Analysis of the Enzymes Associated with 5-Fluorouracil Metabolism in Prostate Cancer Biopsies
Tomoaki Tanaka
26. Microdissection of Gonadal Tissues for Gene Expression Analyses
Anne Jorgensen, Marlene Danner Dalgaard, and Si Brask Sonne
27. Duplex Real Time PCR Assay for Quantifying Mitochondrial DNA Deletions in Laser Microdissected Single Spiral Ganglion Cells
Adam Markaryan, Erik G. Nelson, and Raul Hinojosa
28. Neuronal Type-Specific Gene Expression Profiling and Laser-Capture Microdissection
Charmaine Y. Pietersen, Maribel P. Lim, Laurel Macey, Tsung-Ung W. Woo, and Kai C. Sonntag
29. Region-Specific In Situ Hybridization-Guided Laser Capture Microdissection on Postmortem Human Brain Tissue Coupled with Gene Expression Quantification
Rene Bernard, Sharon Burke, and Ilan A. Kerman
30. UV-Laser Microdissection and mRNA Expression Analysis of Individual Neurons from Post Mortem Parkinson's Disease Brains
Jan Grundemann, Falk Schlaudraff, and Birgit Liss
31. Transcriptome Profiling of Murine Spinal Neurulation Using Laser Capture Microdissection and High-Density Oligonucleotide Microarrays
Shoufeng Cao, Boon-Huat Bay, and George W. Yip
32. Probing the CNS Microvascular Endothelium by Immune-Guided Laser Capture Microdissection Coupled to Quantitative RT-PCR
Nivetha Murugesan, Jennifer Macdonald, Shujun Ge, and Joel S. Pachter
33. Laser Capture Microdissection for Factor VIII-Expressing Endothelial Cells in Cancer Tissues
Tomoatsu Kaneko, Takashi Okiji, Reika Kaneko, Hideaki Suda, and Jacques E. Noer
34. Laser Capture Microdissection and Analysis of Liver Endothelial Cells from Patients with Budd Chiari Syndrome
Selcuk Sozer and Ronald Hoffman
35. Laser Capture Microdissection of Hyperlipidemic/ApoE-/- Mouse Aorta Atherosclerosis
Michael Beer, Sandra Doepping, Markus Hildner, Gabriele Weber, Rolf Grabner, Desheng Hu, Sarajo Kumar Mohanta, Prasad Srikakulapu, Falk Weih, and Andreas J.R. Habenicht
36. Gene Expression Profiling in Laser Microdissected Bone Marrow Megakaryocytes
Kais Hussein
37. Specific RNA Collection from the Rat Endolymphatic Sac by Laser Capture Microdissection (LCM): LCM of Very Small Organ Surrounded by Bony Tissue
Kosuke Akiyama, Takenori Miyashita, Ai Matsubara, and Nozomu Mori
38. The Use of Laser Capture Microdissection on Adult Human Articular Cartilage for Gene Expression Analysis
Naoshi Fukui, Yasuko Ikeda, and Nobuho Tanaka
39. Laser Capture Microdissection of Developing Barley Seeds and cDNA Array Analysis of Selected Tissues
Johannes Thiel, Diana Weier, and Winfriede Weschke
40. Quantitative RT-PCR Gene Expression Analysis of a Laser Microdissected Placenta: An Approach to Study Preeclampsia
Yuditiya Purwosunu, Akihiko Sekizawa, Takashi Okai, and Tetsuhiko Tachikawa
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: July, 2011
Pages: 510
Weight: 1150g
Availability: Available
Subcategories: Genetics
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