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Main description:
It has been more than five years since the appearance of the first edition of Nitric Oxide Protocols. According to Medline, more than 32,500 publications dealing with nitric oxide have appeared in the primary literature in the int- vening time from 1999 to April 2004. These numbers attest to the continuing vitality of a field dealing with a deceptively simple, although extremely int- esting and promiscuous molecule, nitric oxide. Despite its apparent chemical simplicity, nitric oxide continues to amaze, with an extremely wide spectrum of functions and effects. In particular, the last few years have witnessed an explosion of nonclassical effects, involving the direct interaction of nitric - ide and related oxidized products with proteins to yield such novel combi- tions as nitrosylated and nitrated proteins. Another emergent area has been that of NO's clinical applications, including interesting developments related to the virus-mediated expression of nitric oxide synthase and the use of nitric oxide in gene therapy. In Nitric Oxide Protocols, I have gathered chapters dealing with the some of the latest methodologies for the measurement of nitric oxide.
Many of these deal with quantitation in biological fluids or tissues, and they cover a wide spectrum of technologies, including capillary electrophoresis, microcoaxial electrodes, the in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography-mass spectroscopy, in situ hybridization, and real-time polymerase chain reaction.
Contents:
Measurement of Nitric Oxide by Reconversion of Nitrate/Nitrite to NO
Reinhard Berkels, Svenja Purol-Schnabel, and Renate Roesen
High-Resolution Capillary Electrophoresis of Nitrite and Nitrate in Biological Samples
Dmitri Y. Boudko
Nitrite and Nitrate Measurements in Human Urine by Capillary Electrophoresis
Edward Morcos and N. Peter Wiklund
In Vivo Nitric Oxide Measurements Using a Microcoaxial Electrode
Yoshiichiro Kitamura, Hirosuke Kobayashi, Kazuo Tanishita, and Kotaro Oka
Measurement of Exhaled Nitric Oxide
Peter A. Steerenberg and Jan G. C. van Amsterdam
Nitric Oxide Imaging in Neurons Using Confocal Microscopy
Xiaoxiang Zheng, Gangmin Ning, Dihui Hong, and Mu Zhang
Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry
Dimitrios Tsikas
A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts
Hazel Sutherland, Olga Zolle, Roba Khundkar, Alec W. M. Simpson, Jonathan C. Jarvis, and Stanley Salmons
Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization
Olivier Braissant
Real-Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase
Yi Chu and Frank M. Faraci
Dioxygen-Dependent Metabolism of Nitric Oxide
Paul R. Gardner, Anne M. Gardner, and Craig K. Hallstrom
Proteomic Method for Identification of Tyrosine-Nitrated Proteins
Kulwant S. Aulak, Thomas Koeck, John W. Crabb, and Dennis J. Stuehr
Immunohistochemical Detection of S-Nitrosylated Proteins
Andrew J. Gow, Christiana W. Davis, David Munson, and Harry Ischiropoulos
Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernosa for Erectile Dysfunction
Trinity J. Bivalacqua, Weiwen Deng, Hunter C. Champion, Wayne J. G. Hellstrom, and Philip J. Kadowitz
Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer Into the Nucleus Tractus Solitarius in Conscious Rats
Yoshitaka Hirooka and Koji Sakai
Gene Therapy in Cancer Via Use of a Retrovector Having a Tumor Specificity and Expressing Inducible Nitric Oxide Synthase
Masahide Kuroki
Gene Transfer of Nitric Oxide Synthase Via the Use of Adeno-Associated Virus Vectors
Yoshikazu Maeda, Kazuyuki Shimada, and Uichi Ikeda
Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer
Kathleen G. Raman, Richard A. Shapiro, Edith Tzeng, and Melina R. Kibbe
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 258
Weight: 387g
Availability: Available
Subcategories: Biochemistry
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