(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on-off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.
Contents:
Part I. Introduction and Reviews
Calcium-Binding Proteins
Hans J. Vogel, Richard D. Brokx, and Hui Ouyang
Calcium
Robert J. P. Williams
Crystal Structure of Calpain and Insights into Ca2+-Dependent Activation
Zongchao Jia, Christopher M. Hosfield, Peter L. Davies, and John S. Elce
The Multifunctional S100 Protein Family
Claus W. Heizmann
Ca2+ Binding to Proteins Containing g-Carboxyglutamic Acid Residues
Egon Persson
The Caseins of Milk as Calcium-Binding Proteins
Harold M. Farrell, Jr., Thomas F. Kumosinski, Edyth L. Malin, and Eleanor M. Brown
Part II. Calcium-Binding Proteins: Case Studies
Preparation of Recombinant Plant Calmodulin Isoforms
Raymond E. Zielinski
Isolation of Recombinant Cardiac Troponin C
John A. Putkey and Wen Liu
Skeletal Muscle Troponin C: Expression and Purification of the Recombinant Intact Protein and Its Isolated N- and C-Domain Fragments
Joyce R. Pearlstone and Lawrence B. Smillie
Purification of Recombinant Calbindin D9k
Eva Thulin
S100 Proteins: From Purification to Functions
Jean Christophe Deloulme, Gaelh Ouengue Mbele, and Jacques Baudier
Cadherins
Jean-Rene Alattia, Kit I. Tong, Masatoshi Takeichi, and Mitsuhiko Ikura
a-Lactalbumin and (Calcium-Binding) Lysozyme
Katsutoshi Nitta
Recombinant Annexin II Tetramer
Hyoung-Min Kang, Nolan R. Filipenko, Geetha Kassam, and David M. Waisman
Purification and Characterization of ALG-2: A Novel Apoptosis-Linked Ca2+-Binding Protein
Mingjie Zhang and Kevin W.-H. Lo
Crystallization and Structural Details of Ca2+-Induced Conformational Changes in the EF-Hand Domain V1 of Calpain
Miroslaw Cygler, Pawel Grochulski, and Helen Blanchard
Neurocalcin: Role in Neuronal Signaling
Senadhi Vijay-Kumar and Vinod D.Kumar
Crystallization and Structure-Function of Calsequestrin
ChulHee Kang, William R. Trumble, and A. Keith Dunker
Use of Fluorescence Resonance Energy Transfer to Monitor Ca2+-Triggered Membrane Docking of C2 Domains
Eric A. Nalefski and Joseph J. Falke
Ca2+-Binding Mode of the C2A-Domain of Synaptotagmin
Josep Rizo, Josep Ubach, and Jesus Garcia
Study of Calcineurin Structure by Limited Proteolysis
Seun-Ah Yang and Claude Klee
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 356
Weight: 605g
Availability: Available
Subcategories: Biochemistry
From the same series
