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Main description:
The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal-fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis.
Contents:
Part I. Basic Concepts and Techniques
Molecular Cytogenetics in Medicine: An Overview
Yao-Shan Fan
Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets
Larry E. Morrison, Ramesh Ramakrishnan, Teresa M. Ruffalo, and Kim A. Wilber
Labeling Fluorescence In Situ Hybridization Probes for RNA Targets
Ramesh Ramakrishnan and Larry E. Morrison
Basic FISH Techniques and Troubleshooting
Sue Van Stedum and Walter King
Part II. Evolving Techniques and Applications
Chromosome Microdissection
Xin-Yuan Guan
Primed In Situ Labeling
Jorn Koch
Spectral Karyotyping
Jane Bayani and Jeremy A. Squire
Multicolor FISH
Syed M. Jalal and Mark E. Law
Characterization of Constitutional Chromosome Abnormalities by Comparative Genomic Hybridization
Brynn Levy and Kurt Hirschhorn
Color Banding
Vicki L. Hopwood, Christi J. Hada, and Armand B. Glassman
Multicolor Fiber FISH
Jaennelle Kraan, Anne R. M. von Bergh, Karin Kleiverda, Jan-Willem Vaandrager, Ekaterina S. Jordanova, Anton K. Raap, Philip M. Kluin, and Ed Schuuring
Multi-Telomere FISH
Samantha J. L. Knight and Jonathan Flint
Fluorescence Genotyping for Screening Cryptic Telomeric Rearrangements
Laurence Colleaux, Solange Heuertz, Florence Molinari, and Marlene Rio
Microarray CGH, Ben Beheshti, Paul C. Park, Ilan Braude, and Jeremy A. Squire
Part III. Special Applications in Chromosomal Disorders
Molecular Approaches for Delineating Marker Chromosomes
Michael D. Graf and Stuart Schwartz
Interphase FISH for Prenatal Diagnosis of Common Aneuploidies
Baruch Feldman, Ayala Aviram-Goldring, and Mark I. Evans
Prenatal Diagnosis of Chromosomal Abnormalities Using Maternal Blood, Farideh Z. Bischoff, Dianne X. Dang, Dorothy E. Lewis, and Joe LeighSimpson
Preimplantation FISH Diagnosis of Aneuploidies
Yury Verlinsky, Jeanine Cieslak, and Anver Kuliev
Microdeletion Syndromes: Characteristics and Diagnosis
Stuart Schwartz and Michael D. Graf
Molecular Detection of Uniparental Disomy
Wendy P. Robinson
Molecular Cytogenetics in Reproductive Pathology
Helene Bruyere, Evica Rajcan-Separovic, and Dagmar K. Kalousek
Part IV. Special Applications in Oncology
Interphase FISH Studies of Chronic Myeloid Leukemia, Gordon W. Dewald
Chromogenic In Situ Hybridization and FISH in Pathology
Bae-Li Hsi, Sheng Xiao, and Jonathan A. Fletcher
FISH Detection of HER2 Amplification in Breast Cancer
Rosemary E. Mueller and Frances P. O'Malley
Comparative Genomic Hybridization in Cancer Investigations
Marjan M. Weiss, Mario A. J. A. Hermsen, Gerrit A. Meijer, and Paul J. van Diest
Simultaneous Fluorescence Immunophenotyping and FISH on Tumor Cells
Yanming Zhang and Brigitte Schlegelberger
BAC Resource for Molecular Cytogenetics
Xiao-Ning Chen and Julie R. Korenberg
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 424
Weight: 620g
Availability: Available
Subcategories: Genetics
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