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Main description:
The discovery of Epstein-Barr virus (EBV) by Epstein, Achong, and Barr, reported in 1964 (Lancet 1:702-703), was stimulated by Denis Burkitt's rec- nition of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor's etiology (Nature194:232-234, 1962). Since then, molecular and cellular biological and computational technologies have progressed by leaps and bounds. The advent of recombinant DNA technology opened the possibilities of genetic research more than most would have realized. Not only have the molecular tools permitted the analyses of viral mechanisms, but, importantly, they have formed the basis for discerning viral presence and, subsequently, viral involvement in an increasing number of diseases. Though in every field of science the search for further knowledge is likely to be a limitless phenomenon, the distinct goal in EBV research, namely, to gain sufficient insight into the viral-host interaction to be able to intercept the pathogenic process, is beginning to be realized. Epstein-Barr virus research has effectively entered the postgenomic era that began with the sequencing of the first strains, cloned in the mid to late 1980s.
Contents:
I. Genome and Transcript Analyses
Epstein-Barr Virus: The B95-8 Strain Map
Paul J. Farrell
Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCR
Ann L. Kirchmaier
Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids
Ellen Kilger and Wolfgang Hammerschmidt
Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs)
Paul R. Smith. Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous Cells
Kenneth G. Laing, Volker Matys, and Michael J. Clemens
Visualizing EBV Expression Patterns by FISH
Anna Szeles
II. Viral Detection
In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products
Gerald Niedobitek and Hermann Herbst
Phenotype Determination of Epstein-Barr Virus-Infected Cells in Tissue Sections
Hermann Herbst and Gerald Niedobitek
Detection of EBV Infection at the Single-Cell Level: Precise Quantitation of Virus-Infected Cells In Vivo
Gregory J. Babcock, Emily M. Miyashita-Lin, and David A. Thorley-Lawson
Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell Level In Vivo
Lisa L. Decker, Gregory J. Babcock, and David A. Thorley-Lawson
III. Culture Methods
Virus Isolation
Lindsey M. Hutt-Fletcher and Susan M. Turk
Generation of Lymphoblastoid Cell Lines (LCLs)
Teresa Frisan, Victor Levitsky, and Maria Masucci
Cell Cycle Distribution of B-Lymphocytes and Cell Lines
Alison J. Sinclair
Introduction of Plasmid Vectors into Cells Via Electroporation
Ann L. Kirchmaier
Malignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 Gene
Tadamasa Ooka
Transient Gene Expression and MACS Enrichment
Dieter Kube and Martina Vockerodt
In Vitro Assays to Study Epithelial Cell Growth
Christopher W. Dawson and Lawrence S. Young
In Vitro Assays to Study Epithelial Cell Differentiation
Christopher W. Dawson, and Lawrence S. Young
Cell Sensitivity Assays: Quantitative Detection of Apoptotic Cells In Vitro Using the TUNEL Assay
Neil A. Jones and Caroline Dive
Qualitative Detection of Apoptotic Cells Assessed by DNA Fragmentation
Amanda J. McIlwrath
IV. Immune Assays
Regression Assay
Teresa Frisan, Victor Levitsky, and Maria Masucci
Generation of Polyclonal EBV-Specific CTL Cultures and Clones
Victor Levitsky, Teresa Frisan, and Maria Masucci
Determination of Antigen and Fine Peptide Specificity of EBV-Specific CTLs
Victor Levitsky, Teresa Frisan, and Maria Masucci
Limiting Dilution Assay
Teresa Frisan, Victor Levitsky, and Maria Masucci
V. Viral Protein Detection
Antibodies for Detecting EBV Latent Proteins
Martin Rowe, and Anja Mehl
Detection of EBV Latent Proteins by Western Blotting
Martin Rowe and Matthew Jones
Biosynthetic Radiolabeling of Virus Glycoproteins for Immunoprecipitation and Electrophoretic Analysis
Lindsey M. Hutt-Fletcher and Susan M. Turk
VI. Protein-Protein, Protein-DNA, and Protein-RNA Interactions
The Yeast Two-Hybrid Assay to Identify Interacting Proteins
Kenneth M. Izumi
Identification of Transactivation, Repression, and Protein-Protein Interaction Domains Using GAL4-Fusion Proteins
James J.-D. Hsieh and S. Diane Hayward
Magnetic DNA Affinity Purification of a Cellular Transcription Factor
Lothar J. Strobl and Ursula Zimber-Strobl
Screening an Expression Library with a DNA Binding Site
Qin Zhang and Eduardo A. Montalvo
Analysis of DNA Binding Proteins by Mobility S
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 452
Weight: 946g
Availability: Available
Subcategories: Immunology
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