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Main description:
Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.Feature:
Covers biolistic gene transfer into plants, nematodes, or in vitro and in vivo mammalian cells
Includes lab-ready protocols with clear step-by-step direction from leading researchers in the field
Features key tips and implementation advice to guarantee successful results
Back cover:
Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.Contents:
Part I: Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology
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1. Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun
           Caixia Gao and Klaus K. Nielsen
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2. Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery
           Shoko Ueki, Shimpei Magori, Benoît Lacroix, and Vitaly Citovsky
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3. Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues
           Mulpuri Sujatha and K.B.R.S. Visarada
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4. Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-Vacuum Gene Gun (HandyGun)
           Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, and Jari P.T. Valkonen
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5. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies
           Victor Gaba, Moshe Lapidot, and Amit Gal-On
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6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun
           Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn
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Part II: Biolistic DNA Delivery in Nematodes
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7. Biolistic Transformation of Caenorhabditis elegans
           Meltem Isik and Eugene Berezikov
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8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans
           Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher
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9. Biolistic Transformation of Brugia malayi
           Tarig B. Higazi and Thomas R. Unnasch
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Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro
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10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells
           Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens
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11. Biolistic Transfection of Tumor Tissue Samples
           Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang
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12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes
           David F. Steele, Ying Dou, and David Fedida
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13. Biolistic Transfection of Neurons in Organotypic Brain Slices
           John A. O’Brien and Sarah C.R. Lummis
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Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
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14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun
           Meng-Chi Yen and Ming-Derg Lai
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15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery
           Ahmad Jalili
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16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs
           Steve Pascolo
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17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter
           Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz
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18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice
           Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus
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Part V: Biolistic DNA Vaccination in Animal Disease Models
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19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets
           Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller
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20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models
           Gustaf Ahlén, Matti Sällberg, and Lars Frelin
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21. Gene Gun Immunization to Combat Malaria
           Elke S. Bergmann-Leitner and Wolfgang W. Leitner
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22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination
           Toshi Nagata and Yukio Koide
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23. Biolistic DNA Vaccination Against Trypanosoma Infection
           Marianne Bryan, Siobhan Guyach, and Karen A. Norris
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24. Biolistic DNA Vaccination Against Melanoma
           Julia Steitz and Thomas Tüting
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25. Biolistic DNA Vaccination Against Cervical Cancer
           Michal Šmahel
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26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy
           Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe
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27. Safety Assessment of Biolistic DNA Vaccination
           Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek
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Part VI: Related Applications for Biolistic Delivery of Molecules
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28. DiOlistics: Delivery of Fluorescent Dyes into Cells
           Nyssa Sherazee and Veronica A. Alvarez
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29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI)
           Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss
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14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun
           Meng-Chi Yen and Ming-Derg Lai
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15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery
           Ahmad Jalili
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16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs
           Steve Pascolo
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17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter
           Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz
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18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice
           Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus
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Part V: Biolistic DNA Vaccination in Animal Disease Models
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19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets
           Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller
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20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models
           Gustaf Ahlén, Matti Sällberg, and Lars Frelin
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21. Gene Gun Immunization to Combat Malaria
           Elke S. Bergmann-Leitner and Wolfgang W. Leitner
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22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination
           Toshi Nagata and Yukio Koide
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23. Biolistic DNA Vaccination Against Trypanosoma Infection
           Marianne Bryan, Siobhan Guyach, and Karen A. Norris
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24. Biolistic DNA Vaccination Against Melanoma
           Julia Steitz and Thomas Tüting
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25. Biolistic DNA Vaccination Against Cervical Cancer
           Michal Šmahel
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26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy
           Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe
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27. Safety Assessment of Biolistic DNA Vaccination
           Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek
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Part VI: Related Applications for Biolistic Delivery of Molecules
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28. DiOlistics: Delivery of Fluorescent Dyes into Cells
           Nyssa Sherazee and Veronica A. Alvarez
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29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI)
           Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss
           Mulpuri Sujatha and K.B.R.S. Visarada
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: October, 2012
Pages: 400
Weight: 1000g
Availability: Not available (reason unspecified)
Subcategories: Genetics
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