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Main description:
In recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized, and much effort is being directed toward the development of alternative models, utilizing mostly mammalian cells in culture but also non-mammalian model systems. In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology, with an emphasis on mammalian cell culture systems. Opening with a section on methodologies for preparing several cellular systems of variable complexity, amenable for in vitro neurotoxicological studies, the thorough volume continues with coverage of methods to measure cellular death and major mechanisms, methods for assessing mechanisms of nervous system cell toxicity related to impairment of cell signaling, while a final section illustrates additional methods for assessing important nervous system processes such as cell proliferation, neuritogenesis, and synaptogenesis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, In Vitro Neurotoxicology: Methods and Protocols serves researchers with an interest in assessing or characterizing the potential neurotoxicity of environmental contaminants, drugs, or other chemicals.
Contents:
1. In Vitro Neurotoxicology: An Introduction
Lucio G. Costa, Gennaro Giordano, and Marina Guizzetti
Part I: Model Systems
2. Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies
Gennaro Giordano and Lucio G. Costa
3. Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures
Evelyn Tiffany-Castiglioni, Yongchang Qian, Rola Barhoumi, and Ying Zheng
4. Microglia Cell Culture: A Primer for the Novice
Anke Witting and Thomas Moeller
5. Neural Stem Cells for Developmental Neurotoxicity Studies
Roshan Tofighi, Michaela Moors, Raj Bose, Wan Norhamidah Wan Ibrahim, and Sandra Ceccatelli
6. Preparation, Maintenance, and Use of Serum-Free Aggregating Brain Cell Cultures
Paul Honegger, Antoinette Defaux, Florianne Monnet-Tschudi, and Marie-Gabrielle Zurich
7. Neurospheres as a Model for Developmental Neurotoxicity Testing
Ellen Fritsche, Kathrin Gassmann, and Timm Schreiber
8. Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures
Pamela J. Lein, Christopher D. Barnhart, and Isaac N. Pessah
9. Glia-Neuron Sandwich Co-Cultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity
Mariaserena Boraso and Barbara Viviani
10. In Vitro Models to Study the Blood Brain Barrier
Hilary Vernon, Katherine Clark, and Joseph P. Bressler
Part II: Cell Death
11. Measurements of Cell Death in Neuronal and Glial Cells
Gennaro Giordano, Sungwoo Hong , Elaine M. Faustman, and Lucio G. Costa
12. Measurements of Neuronal Apoptosis
Gennaro Giordano and Lucio G. Costa
13. Measurement of Isoprostanes as Markers of Oxidative Stress
Dejan Milatovic, Thomas J. Montine, and Michael Aschner
14. Assessment of Glutathione Homeostasis
Gennaro Giordano, Collin C. White, and Lucio G. Costa
15. Assessing Neuronal Bioenergetic Status
Stephanie L.H. Zeiger, Jeannette N. Stankowski, and BethAnn McLaughlin
Part III: Signaling in Neurotoxicity
16. Fluorescent Assessment of Intracellular Calcium in Individual Cells
David P. Cox, Collin C. White, and Terrance J. Kavanagh
17. Homeostatic Regulation of Glutamate Neurotransmission in Primary Neuronal Cultures
Victor Briz and Cristina Sunol
18. Detection of Nitric Oxide Formation in Primary Neural Cells and Tissues
Ronald B. Tjalkens, David L. Carbone, and Guoyao Wu
19. JNK3-Mediated Apoptotic Cell Death in Primary Dopaminergic Neurons
Won-Seok Choi, Heather M Klintworth, and Zhengui Xia
20. Measurement of Proteasomal Dysfunction in Cell Models of Dopaminergic Degeneration
Faneng Sun, Anumantha G. Kanthasamy, and Arthi Kanthasamy
21. Cell Signaling and Neurotoxicity: Protein Kinase C In Vitro and In Vivo
Prasada Rao S. Kodavanti
22. Cell Signaling and Neurotoxicity: 3H-Arachidonic Acid Release (Phospholipase A2) in Cerebellar Granule Neurons
Prasada Rao S. Kodavanti
Part IV: Other End-Points
23. Quantitative Assessment of Neurite Outgrowth in PC12 Cells
Joshua A. Harrill and William R. Mundy
24. Measurements of Astrocyte Proliferation
Marina Guizzetti, Terrance J. Kavanagh, and Lucio G. Costa
25. Quantification of Synaptic Structure Formation in Co-Cultures of Astrocytes and Hippocampal Neurons
Pamela J. Roque, Marina Guizzetti, Gennaro Giordano, and Lucio G. Costa
26. Volume Measurements in Cultured Primary Astrocytes
Michael Aschner
27. Assessment of Cholesterol Homeostasis in Astrocytes and Neurons
Jing Chen, Lucio G. Costa, and Marina Guizzetti
28. Dose-Response or Dose-Effect Curves in In Vitro Experiments and Their Use to Study Combined Effects of Neurotoxicants
Matteo Goldoni and Sara Tagliaferri
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: July, 2011
Pages: 427
Weight: 1070g
Availability: Available
Subcategories: Neurology, Neuroscience, Pathology, Pharmacology
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