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Main description:
In the past several years significant attention has been given to the analysis of the properties and functions of lateral microdomains (rafts) in biological membranes. As described in the overview chapter of this book, as well as in the introductions to many of the other chapters, there are many fundamental un- swered questions concerning the composition, structure, dynamics, and even the very existence of these membrane rafts. Therefore, a variety of sophisticated techniques have been used to study intact cell membranes, as well as model s- tems composed of specific lipids and proteins thought to be in rafts. The analyses of biological membranes have provided detailed data on intact microdomains, and the complementary studies of model systems have given critical insights on the roles of specific lipid-lipid and lipid-protein interactions in raft formation and function. The chapters in this book provide detailed information on modern methods that are currently being employed to study lipid rafts.
Feature:
In-depth examination of sophisticated techniques used to study intact cell membranes
Proven, detailed biochemical, biophysical, and computational methods and protocols
Contents:
TABLE OF CONTENTS
'Lipid Rafts'
Volume for Methods in Molecular Biology
Chapters:
1. 'Overview of Membrane Rafts' by Thomas J. McIntosh, Duke University
Medical Center.
2. 'Analysis of Raft Affinity of Membrane Proteins by Detergent
Insolubility' by Deborah A. Brown, SUNY Stony Brook
3. 'Non-detergent Isolation of Rafts' by Mehul Shah and Pravin Sehgal,
New York Medical College.
4. 'Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes
Using Tempo' by Omar Bakht and Erwin London, SUNY Stony Brook
5. 'Using Monomolecular Films to Characterize Lipid Lateral
Interactions' by Rhoderick E. Brown and Howard L. Brockman, University
of Minnesota
6. 'Electro-formation and Fluorescence Microscopy of Giant Vesicles
with Coexisting Liquid Phases' by Sarah Veatch, University of British
Columbia.
7. 'Fluorescence Correlation Spectroscopy' by Kirsten Bacia and Petra
Schwille, Dresden University.
8. 'Multiphoton Laser Scanning Microscopy and Spatial Analysis of
Dehydroergosterol Distribution on Plasma Membrane of Living Cells' by
Avery McIntosh, Barbara Atshaves, Adalberto M. Gallegos, Ann B. Kier,
Friedhelm Schroeder, Hai Xu, Weimin Zhang, Suojin Wang, and Jyh-Charn
Liu, Texas A&M University.
9. 'NMR Detection of Lipid Domains' by Klaus Gawrisch, NIAAA, NIH
10. 'Lateral Diffusion Coefficients of Raft Lipids from Pulsed Field
Gradient NMR' by Greger Oradd and Goran Lindblom, Umea University.
11. 'Saturation-Recovery EPR Discrimination by Oxygen Transport Method
for Characterizing Membrane Domains' by Witold K. Subczynski, Justyna
Widomska, Anna Wisniewska, and Akihiro Kusumi, Medical College
Wisconsin.
12. 'Plasmon-Waveguide Resonance Spectroscopy Studies of Lateral
Segregation in Solid-Supported Proteolipid Bilayers' by Zdzislaw
Salamon, Savitha Devanathan, and Gordon Tollin, University of Arizona.
13. 'Fluorescence Recovery after Photobleaching Studies ofLipid Rafts'
by Anne Kenworthy, Vanderbilt
14. 'Single Molecule Tracking' by Marija Vrljic, Stefanie Y. Nishimura,
and W. E. Moerner, Stanford University
15. 'X-ray Diffraction to Determine the Thickness of Raft and Non-raft
Bilayers' by Thomas J. McIntosh, Duke University Medical Center.
16. 'Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains'
by Jeremy Pencer, Thaila T. Mills, Norbert Kucerka, Mu-Ping Nieh, and
John Katsaras, Canadian National Research Council
17. 'Exploring Membrane Domains Using Native Membrane Sheets and
Transmission Electron Microscopy' by Bridget S. Wilson, Janet R.
Pfeiffer, Mary Ann Raymond-Stintz, Diane Lidke, Nicholas Andrews, Jun
Zhang, Wenxia Yin, Stanly Steinberg, and Janet M. Oliver, University of
New Mexico.
18. 'Atomic Force Microscopy of Lipid Domains in Supported Model
Membranes' by Alan Burns, Sandia National Laboratories.
19. 'Atomistic and Coarse-grained Computer Simulations of Raft-like
Lipid Mixtures' by Sagar A. Pandit and Larry Scott, Illinois Institute
of Technology
20. 'A Microscopic Model Calculation of the Phase Diagram of Ternary
Mixtures of Cholesterol and Saturated and Unsaturated Phospholipids' by
R. Elliot, I. Szleifer, and M. Schick, UC Santa Barbara, Purdue
University, and University of Washington.
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: July, 2007
Pages: 326
Weight: 672g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry
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