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Main description:
Peptide nucleic acids (PNAs) have now existed for slightly more than ten years, with the interest in and applications of this pseudopeptide DNA mimic steadily increasing during the entire period. PNAs have rapidly attracted the attention of scientists from a diversity of fields ranging from (bio)organic and biophysical chemistry to prebiotic evolution, and from molecular biology to genetic diagnostics and drug development. Many of the applications take advantage of the unique properties of PNA-an uncharged pseudopeptide-that distinguish this DNA mimic from more traditional DNA analogs. Rather than trying to create a comprehensive collection of all published methods and protocols involving PNA-many of which have not yet been validated- I have decided to concentrate on select protocols that are either very well established by several groups around the world, such as PCR-clamping and in situ hybridization, or on new methods that may have broader future impact. Basic methods for PNA oligomer synthesis and analyses have also been included. I am very grateful to those friends and colleagues who have enthusiastically contributed their work, discussions, and writing, and thereby made this book possible. Peter E. Nielsen v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix IINTRODUCTION 1 PNA Technology Peter E. Nielsen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II CHEMISTRY 2 Solid Phase Synthesis of PNA Oligomers Frederik Beck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 Synthesis of PNA-Peptide Conjugates Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . . 43 4 Parallel Synthesis of PNA-Peptide Conjugate Libraries Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . .
Contents:
Part I. Introduction
PNA Technology
Peter E. Nielsen
Part II. Chemistry
Solid Phase Synthesis of PNA Oligomers
Frederik Beck
Synthesis of PNA-Peptide Conjugates
Satish Kumar Awasthi and Peter E. Nielsen
Parallel Synthesis of PNA-Peptide Conjugate Libraries
Satish Kumar Awasthi and Peter E. Nielsen
Thermodynamics of PNA Interactions with DNA and RNA
Tommi Ratilainen and Bengt Norden
Part III. PNA Technology
Measurement of PNA Binding to Double-Stranded DNA
Thomas Bentin, Georg I. Hansen, and Peter E. Nielsen
PNA-Mediated Immobilization of Supercoiled DNA
Thomas Bentin and Peter E. Nielsen
PNA Openers and Their Applications
Vadim V. Demidov and Maxim D. Frank-Kamenetskii
Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes
Miaomiao Wang and Bruce A. Armitage
Part IV. Diagnostics
PNA-Mediated PCR Clamping: Applications and Methods
Deborah G. Murdock and Douglas C. Wallace
PNA Clamping Techniques for the Determination of Oncogene Mutations
Marcus Schuermann and Moira Behn. PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic Analysis
Brett Williams, Henrik Stender, and James M. Coull
Detection of Point Mutations Using PNA-Containing Electrophoresis Matrices
Gabor L. Igloi
Part V. Antisense and Antigene
Lipid-Mediated Introduction of Peptide Nucleic Acids Into Cells
Dwaine A. Braasch and David R. Corey
Synthesis of Cell-Penetrating Peptide-PNA Constructs
Margus Pooga, Ursel Soomets, Tamas Bartfai, and UElo Langel
Antisense Inhibition of Bacterial Gene Expression and Cell Growth
Liam Good
In Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement Loops
Niels Erik Mollegaard and Peter E. Nielsen
Part VI.Animal Studies
In Vitro and in Vivo Studies on Pharmacokinetics and Metabolism of PNA Constructs in Rodents
Edward Kristensen
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 288
Weight: 940g
Availability: Available
Subcategories: Biochemistry
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