(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response. In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers provide concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to dissect human disease. These methodologies are the toolbox for revolutionizing drug development and cell-level biochemical understanding of human disease processes. Beginning with a section on protein-detecting analytical microarrays, the volume continues with sections covering antigen microarrays for immunoprofiling, protein function microarrays, the validation of candidate targets, proteomic libraries, as well as signal detection strategies and data analysis techniques. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Protein Microarray for Disease Analysis: Methods and Protocols serves as a solid framework to aid scientists in understanding how protein microarray technology is presently developing and how it can be applied to transform our analysis of human disease.
Contents:
Part I: Protein-Detecting Analytical Microarrays
1. Detecting and Quantifying Multiple Proteins in Clinical Samples in High-Throughput Using Antibody Microarrays
Tanya Knickerbocker and Gavin MacBeath
2. Analysis of Serum Protein Glycosylation with Antibody-Lectin Microarray for High-Throughput Biomarker Screening
Chen Li and David M. Lubman
3. Antibody Suspension Bead Arrays
Jochen M. Schwenk and Peter Nilsson
4. Reverse Protein Arrays Applied to Host-Pathogen Interaction Studies
Victor J. Cid, Ekkehard Kauffmann, and Maria Molina
5. Identification and Optimization of DNA Aptamer Binding Regions Using DNA Microarrays
Nicholas O. Fischer and Theodore M. Tarasow
6. Recombinant Lectin Microarrays for Glycomic Analysis
Daniel C. Propheter, Ku-Lung Hsu, and Lara K. Mahal
Part II: Antigen Microarrays for Immunoprofiling
7. Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection
Persis P. Wadia, Bita Sahaf, and David B. Miklos
8. SPOT Synthesis as a Tool to Study Protein-Protein Interactions
Dirk F.H. Winkler, Heiko Andresen, and Kai Hilpert
9. Native Antigen Fractionation Protein Microarrays for Biomarker Discovery
Robert J. Caiazzo, Jr., Dennis J. O'Rourke, Timothy J. Barder, Bryce P. Nelson, and Brian C.-S. Liu
10. Immunoprofiling Using NAPPA Protein Microarrays
Sahar Sibani and Joshua LaBaer
Part III: Protein Function Microarrays
11. High-Throughput Mammalian Two-Hybrid Screening for Protein-Protein Interactions Using Transfected Cell Arrays (CAPPIA)
Andrea Fiebitz and Dominique Vanhecke
12. Protein-Protein Interactions: An Application of Tus-Ter Mediated Protein Microarray System
Kalavathy Sitaraman and Deb K. Chatterjee
13. Kinase Substrate Interactions
Michael G. Smith, Jason Ptacek, and Michael Snyder
14. A Functional Protein Microarray Approach to Characterizing Posttranslational Modifications on Lysine Residues
Jun Seop Jeong, Hee-Sool Rho, and Heng Zhu
Part IV: Strategies for Validation of Candidate Targets
15. Multiplexed Detection of Antibodies Using Programmable Bead Arrays
Karen S. Anderson
16. A Co-Precipitation-Based Validation Methodology for Interactions Identified Using Protein Microarrays
Ovidiu Marina, Jonathan S. Duke-Cohan, and Catherine J. Wu
Part V: Generation of Proteomic Libraries
17. Development of Expression-Ready Constructs for Generation of Proteomic Libraries
Charles Yu, Kenneth H. Wan, Ann S. Hammonds, Mark Stapleton, Joseph W. Carlson, and Susan E. Celniker
Part VI: Detection Methods
18. Reverse Phase Protein Microarrays: Fluorometric and Colorimetric Detection
Rosa I. Gallagher, Alessandra Silvestri, Emanuel F. Petricoin III, Lance A. Liotta, and Virginia Espina
19. Foerster Resonance Energy Transfer Methods for Quantification of Protein-Protein Interactions on Microarrays
Michael Schaferling and Stefan Nagl
20. Label-Free Detection with Surface Plasmon Resonance Imaging
Christopher Lausted, Zhiyuan Hu, and Leroy Hood
Part VII: Data Analysis Techniques for Protein Function Microarrays
21. Data Processing and Analysis for Protein Microarrays
David S. DeLuca, Ovidiu Marina, Surajit Ray, Guang L. Zhang, Catherine J. Wu, and Vladimir Brusic
22. Database Resources for Proteomics-Based Analysis of Cancer
Guang Lan Zhang, David S. DeLuca, and Vladimir Brusic
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: March, 2011
Pages: 386
Weight: 925g
Availability: Available
Subcategories: Genetics
From the same series
