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Main description:
Studies related to recombinant gene expression have brought new advance such as the emergence of the “omics” technologies. While Escherichia coli, Sacharomyces cerevisiae and insect cells continue to be the dominant production platforms of recombinant proteins. In Recombinant Gene Expression: Review and Protocols, Third Edition, expert researchers in the field detail many of the methods now commonly used to study recombinant gene expression. These include methods and techniques for bacteria, lower eukaryotes, fungi, plants and plant cells, and animals and animal cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Recombinant Gene Expression: Review and Protocols, Third Edition seeks to aid scientists in the further study of this crucially important research into recombinant gene expression.
Feature:
Contains new protocols and topics not yet covered
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
Studies related to recombinant gene expression have brought new advance such as the emergence of the “omics” technologies. While Escherichia coli, Sacharomyces cerevisiae and insect cells continue to be the dominant production platforms of recombinant proteins. In Recombinant Gene Expression: Review and Protocols, Third Edition, expert researchers in the field detail many of the methods now commonly used to study recombinant gene expression. These include methods and techniques for bacteria, lower eukaryotes, fungi, plants and plant cells, and animals and animal cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Recombinant Gene Expression: Review and Protocols, Third Edition seeks to aid scientists in the further study of this crucially important research into recombinant gene expression.
Contents:
Table of Contents
“Recombinant Gene Expression: Reviews and Protocols, Third Edition”
Argelia Lorence (Editor)
Name of corresponding author(s) is underlined
General Aspects
1) Using Folding Promoting Agents in Recombinant Protein Production: A Review
Beatrix Fahnert
2) Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry
Brett J. Savary and Prasanna Vasu
3) A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene Expression
María de la Cruz Muñoz-Centeno, Gonzalo Millán-Zambrano and Sebastián Chavez
4) Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteins
Maureen C. Dolan, Giuliana Medrano, Anthony McMickle, and Carole L. Cramer
Prokaryotes
5) Heat-shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli
Christos A. Kyratsous and Christos A. Panagiotidis
6) The use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella
Ferenc Vonderviszt, Rachel Sajo, Jozsef Dobo, Peter Zavodoszky
7) Optimization of Purification Protocols Based on the Step-by-step Monitoring of the Protein Aggregates in the Soluble Fractions
Ario De Marco
8) Heterologous Protein Expression by Lactococcus lactis
Julio Villatoro-Hernández, Oscar Paul Kuipers, Odila Saucedo-Cárdenas and Roberto Montes de Oca Luna
9) An Extended Suite of Genetic Tools for Use in Bacteria of the Halomonadaceae: An Overview
Monserrat Argandoña, Carmen Vargas, Javier Rodríguez-Moya, Mercedes Reina-Bueno, Manuel Salvador and Joaquin J. Nieto
10) Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125
Valentina Rippa, Rosanna Papa, Maria Giuliani, Cinzia Pezzella, Ermenegilda Parrilli, Maria Luisa Tutino, Gennaro Marino, and Angela Duilio
11) A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125
Maria Giuliani, Ermenegilda Parrilli , Cinzia Pezzella,Valentina Rippa, Angela Duilio, Gennaro Marino and Maria Luisa Tutino
12) A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein Interaction
Jumei Zeng and Zheng-Guo He
13) Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatus
Nadine Katzke, René Bergmann, Karl-Erich Jaeger Thomas Drepper
14) Plasmid DNA Production for Therapeutic Applications
Alvaro Lara and Octavio T. Ramirez
Lower eukaryotes
15) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review
Tomoaki Niimi
16) Expression of Multi-subunit Proteins in Leishmania tarentolae
Marisa Sugino and Tomoaki Niimi
Fungi
17) Recombinant Protein Production in Yeasts
Diethard Mattanovich, Paola Branduardi, Laura Dato, Brigitte Gasser, Michael Sauer and Danilo Porro
18) Yeasts as Tool for Heterologous Gene Expression
Raja Mokdad-Gargouri, Salma Abdelmoula-Soussi, Nadia Hadiji-Abbès, Ines Yacoubi-Hadj Amor, Istabrak Borchani-Chabchoub and Ali Gargouri
19) The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes
Simona Florea, Caroline Machado, Kalina Andreeva, and Christopher L. Schardl
20) Aptamer-regulated Expression of Essential Genes in Yeast
Beatrix Suess, Karl-Dieter Entian, Peter Kötter and Julia E. Weigand
21) Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris
Prasanna Vasu, Stefan Bauer, and Brett J. Savary
22) A Thiamine-regulatable Epitope-tagged Protein Expression System in Fission Yeast Tiina Tamm
23) Heterologous Gene Expression by Chromosomal Integration in Fission Yeast
Akihisa Matsuyama and Minoru Yoshida
24) Genetic Engineering of Industrial Strains of Saccharomyces cerevisiae
Sylvie Le Borgne Le Gall
Plants and Plant Cells
25) Recombinant Protein Production in Plants: Challenges and Solutions
Elizabeth E. Hood and Deborah V. Vicuña Requesens
26) A Novel Plant Cell Bioproduction Platform for High-yield Secretion of Recombinant Proteins
Jianfeng Xu and Marcia J. Kieliszewski
27) Super-promoter:TEV, a Powerful Gene Expression System for Tobacco Hairy Roots
Luis Ñopo, Bonnie J. Woffenden, Deborah Reed, Scott Buswell, Chenming Zhang, and Fabricio Medina-Bolivar
28) Bioseparation of Recombinant Proteins from Plant Extract with Hydrophobin Fusion Technology
Jussi J Joensuu, Andrew J. Conley, Markus B. Linder, Rima Menassa
29) Quality Assessment of Recombinant Proteins Produced in Plants
Giuliana Medrano, Maureen C. Dolan, Jose Condori, David N. Radin, and Carole L. Cramer
30) Cell-free Protein Synthesis as a Promising Expression System for Recombinant Proteins
Xumeng Ge and Jianfeng Xu
Animals and Animal Cells
31) The use of Bacterial Artificial Chromosomes for Recombinant Protein Production in Mammalian Cell Lines
Leander Blaas, Monica Musteanu, Beatrice Grabner, Robert Eferl, Anton Bauer, and Emilio Casanova
32) Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and Authenticity
Lyne Jossé, Mark Smales, and Mick F. Tuite
33) High-throughput Baculovirus Expression in Insect Cells Richard B. Hitchman Robert D. Possee, and Linda A. King
34) Recombinant Protein Expression in Milk of Livestock Species
Zsuzsanna Bősze and Laszló Hiripi
11) A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125
Maria Giuliani, Ermenegilda Parrilli , Cinzia Pezzella,Valentina Rippa, Angela Duilio, Gennaro Marino and Maria Luisa Tutino
12) A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein Interaction
Jumei Zeng and Zheng-Guo He
13) Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatus
Nadine Katzke, René Bergmann, Karl-Erich Jaeger Thomas Drepper
14) Plasmid DNA Production for Therapeutic Applications
Alvaro Lara and Octavio T. Ramirez
Lower eukaryotes
15) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review
Tomoaki Niimi
16) Expression of Multi-subunit Proteins in Leishmania tarentolae
Marisa Sugino and Tomoaki Niimi
Fungi
17) Recombinant Protein Production in Yeasts
Diethard Mattanovich, Paola Branduardi, Laura Dato, Brigitte Gasser, Michael Sauer and Danilo Porro
18) Yeasts as Tool for Heterologous Gene Expression
Raja Mokdad-Gargouri, Salma Abdelmoula-Soussi, Nadia Hadiji-Abbès, Ines Yacoubi-Hadj Amor, Istabrak Borchani-Chabchoub and Ali Gargouri
19) The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes
Simona Florea, Caroline Machado, Kalina Andreeva, and Christopher L. Schardl
20) Aptamer-regulated Expression of Essential Genes in Yeast
Beatrix Suess, Karl-Dieter Entian, Peter Kötter and Julia E. Weigand
21) Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris
Prasanna Vasu, Stefan Bauer, and Brett J. Savary
22) A Thiamine-regulatable Epitope-tagged Protein Expression System in Fission Yeast Tiina Tamm
23) Heterologous Gene Expression by Chromosomal Integration in Fission Yeast
Akihisa Matsuyama and Minoru Yoshida
24) Genetic Engineering of Industrial Strains of Saccharomyces cerevisiae
Sylvie Le Borgne Le Gall
Plants and Plant Cells
25) Recombinant Protein Production in Plants: Challenges and Solutions
Elizabeth E. Hood and Deborah V. Vicuña Requesens
26) A Novel Plant Cell Bioproduction Platform for High-yield Secretion of Recombinant Proteins
Jianfeng Xu and Marcia J. Kieliszewski
27) Super-promoter:TEV, a Powerful Gene Expression System for Tobacco Hairy Roots
Luis Ñopo, Bonnie J. Woffenden, Deborah Reed, Scott Buswell, Chenming Zhang, and Fabricio Medina-Bolivar
28) Bioseparation of Recombinant Proteins from Plant Extract with Hydrophobin Fusion Technology
Jussi J Joensuu, Andrew J. Conley, Markus B. Linder, Rima Menassa
29) Quality Assessment of Recombinant Proteins Produced in Plants
Giuliana Medrano, Maureen C. Dolan, Jose Condori, David N. Radin, and Carole L. Cramer
30) Cell-free Protein Synthesis as a Promising Expression System for Recombinant Proteins
Xumeng Ge and Jianfeng Xu
Animals and Animal Cells
31) The use of Bacterial Artificial Chromosomes for Recombinant Protein Production in Mammalian Cell Lines
Leander Blaas, Monica Musteanu, Beatrice Grabner, Robert Eferl, Anton Bauer, and Emilio Casanova
32) Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and Authenticity
Lyne Jossé, Mark Smales, and Mick F. Tuite
33) High-throughput Baculovirus Expression in Insect Cells Richard B. Hitchman Robert D. Possee, and Linda A. King
34) Recombinant Protein Expression in Milk of Livestock Species
Zsuzsanna Bősze and Laszló Hiripi
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: December, 2011
Pages: 639
Weight: 1436g
Availability: Not available (reason unspecified)
Subcategories: Genetics
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