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Main description:
New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems. For example, proteomic, genomic, and molecular approaches have afforded us unprecedented insight into the complex protein interaction networks at work on the telomere chromatin and the detailed information regarding telomere dynamics in response to stress or stimuli. Telomeres and Telomerase: Methods and Protocols, Second Edition builds upon the telomerase assays featured in the popular first edition to encompass many different assays that allow investigators to query the function of telomere proteins and the responses of the telomere DNA, including detailed examinations of biochemical, molecular, and proteomic approaches. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Telomeres and Telomerase: Methods and Protocols, Second Edition serves as an ideal, up-to-date guide for investigators further pursing this vital field of study.
Contents:
1. Introduction to Telomeres and Telomerase
Zhou Songyang
2. Analysis of Average Telomere Length in Cultured Human Cells
Dan Liu
3. Telomere Length Analysis by Quantitative Fluorescent In situ Hybridization (Q-FISH)
Isabelle Ourliac-Garnier and Arturo Londono-Vallejo
4. Telomere Strand-Specific Length Analysis by Fluorescent In situ Hybridization (Q-CO-FISH)
Isabelle Ourliac-Garnier and Arturo Londono-Vallejo
5. Telomere G-Overhang Length Measurement Method 1: The DSN Method
Yong Zhao, Jerry W. Shay, and Woodring E. Wright
6. Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA
Hidetoshi Tahara
7. Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-rich Fill-In
Yong Zhao, Jerry W. Shay, and Woodring E. Wright
8. G-Quadruplex Structures and G-Quadruplex-Interactive Compounds
Raveendra I. Mathad and Danzhou Yang
9. Analysis of Yeast Telomerase by Primer Extension Assays
Min Hsu and Neal F. Lue
10. Telomeric Repeat Amplification Protocol (TRAP): Measuring the Activity of the Telomerase
Huawei Xin
11. CO-FISH, COD-FISH, ReD-FISH, SKY-FISH
Eli S. Williams, Michael N. Cornforth, Edwin H. Goodwin, and Susan M. Bailey
12. Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques
Eladio Abreu, Rebecca M. Terns, and Michael P. Terns
13. Cytogenetic Analysis of Telomere Dysfunction
Asha Multani and Sandy Chang
14. Probing the Telomere Damage Response
Relcha Rai and Sandy Chang
15. Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)
Wenbin Ma
16. Studying of Telomeric Protein-Protein Interactions by Bi-Molecular Fluorescence Complementation (BiFC) and Peptide Array-Based Assays
Wenbin Ma, Hyeung Kim, and Zhou Songyang
17. Human Telomere POT1-TPP1 Complex and Its Role in Telomerase Activity Regulation
Feng Wang and Ming Lei
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: April, 2011
Pages: 201
Weight: 624g
Availability: Available
Subcategories: Biochemistry
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